The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T2 relaxivity caused by the chemical exchange (R2ex) between amine protons and water protons was 0.10 mM−1 s−1 at 37 °C. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.
|Number of pages||6|
|Journal||Magnetic Resonance Imaging|
|Publication status||Published - 2022 Nov|
Bibliographical noteFunding Information:
This study is supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019 R1I1A1A01064082 to CGJ).
© 2022 Elsevier Inc.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Radiology Nuclear Medicine and imaging