MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

Seung Hyun Yang; Yuna Choi; Mirae Park; Hye Young Son; Yong Min Huh; Chan Gyu Joo

doi:10.1016/j.mri.2022.08.015

MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

Seung Hyun Yang, Yuna Choi, Mirae Park, Hye Young Son, Yong Min Huh, Chan Gyu Joo

Department of Radiology

Research output: Contribution to journal › Article › peer-review

Abstract

The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T₂ relaxivity caused by the chemical exchange (R_2ex) between amine protons and water protons was 0.10 mM⁻¹ s⁻¹ at 37 °C. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.

Original language	English
Pages (from-to)	189-194
Number of pages	6
Journal	Magnetic Resonance Imaging
Volume	93
DOIs	https://doi.org/10.1016/j.mri.2022.08.015
Publication status	Published - 2022 Nov

Bibliographical note

Funding Information:
This study is supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019 R1I1A1A01064082 to CGJ).

Publisher Copyright:
© 2022 Elsevier Inc.

All Science Journal Classification (ASJC) codes

Biophysics
Biomedical Engineering
Radiology Nuclear Medicine and imaging

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mri.2022.08.015

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title = "MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma",

abstract = "The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T2 relaxivity caused by the chemical exchange (R2ex) between amine protons and water protons was 0.10 mM−1 s−1 at 37 °C. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.",

author = "Yang, {Seung Hyun} and Yuna Choi and Mirae Park and Son, {Hye Young} and Huh, {Yong Min} and Joo, {Chan Gyu}",

note = "Funding Information: This study is supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019 R1I1A1A01064082 to CGJ). Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

year = "2022",

month = nov,

doi = "10.1016/j.mri.2022.08.015",

language = "English",

volume = "93",

pages = "189--194",

journal = "Magnetic Resonance Imaging",

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T1 - MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

AU - Yang, Seung Hyun

AU - Choi, Yuna

AU - Park, Mirae

AU - Son, Hye Young

AU - Huh, Yong Min

AU - Joo, Chan Gyu

N1 - Funding Information: This study is supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019 R1I1A1A01064082 to CGJ). Publisher Copyright: © 2022 Elsevier Inc.

PY - 2022/11

Y1 - 2022/11

N2 - The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T2 relaxivity caused by the chemical exchange (R2ex) between amine protons and water protons was 0.10 mM−1 s−1 at 37 °C. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.

AB - The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T2 relaxivity caused by the chemical exchange (R2ex) between amine protons and water protons was 0.10 mM−1 s−1 at 37 °C. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.

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MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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