Recent evidence suggested an involvement of homeobox genes in tumorigenesis. Here we investigated whether one of homeobox-containing genes, Msx1, might be involved in the regulation of cell proliferation and cell cycle using Msx1 overexpressing human ovarian cancer cell line, OVCAR3. Overexpression of Msx1 in OVCAR3 cells inhibited cell proliferation by markedly increasing the length of the G1 phase of the cell cycle over control cells. Consistent with this result, dramatic suppression of cyclins D1, D3, E, cyclin-dependent kinase 4, c-Jun, and Rb was observed. Elevated expression of genes involved in the growth arrest and apoptosis (GADD153 and apoptotic cystein protease MCH4) and suppression of proliferation associated protein gene (PAG) in Msx1-overexpressing cells by cDNA expression array analysis provide further evidence for a potential repressor function of Msx1 in cell cycle progression.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2001|
Bibliographical noteFunding Information:
1This work was supported by G7 strategic fund (99-G-08-02-1), SRC fund from KOSEF, and SBRI fund (C99-001-2) from Samsung Biomedical Research Institute. 2To whom correspondence and requests for reprints should be addressed. Fax: 82-2-3410-0044. E-mail: firstname.lastname@example.org.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology