Msx1 gene overexpression induces g1 phase cell arrest in human ovarian cancer cell line OVCAR31

Jinhee Park, Kyoungsook Park, Sunghoon Kim, Je Ho Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent evidence suggested an involvement of homeobox genes in tumorigenesis. Here we investigated whether one of homeobox-containing genes, Msx1, might be involved in the regulation of cell proliferation and cell cycle using Msx1 overexpressing human ovarian cancer cell line, OVCAR3. Overexpression of Msx1 in OVCAR3 cells inhibited cell proliferation by markedly increasing the length of the G1 phase of the cell cycle over control cells. Consistent with this result, dramatic suppression of cyclins D1, D3, E, cyclin-dependent kinase 4, c-Jun, and Rb was observed. Elevated expression of genes involved in the growth arrest and apoptosis (GADD153 and apoptotic cystein protease MCH4) and suppression of proliferation associated protein gene (PAG) in Msx1-overexpressing cells by cDNA expression array analysis provide further evidence for a potential repressor function of Msx1 in cell cycle progression.

Original languageEnglish
Pages (from-to)1234-1240
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume281
Issue number5
DOIs
Publication statusPublished - 2001 Jan 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this