MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: Role in corneal angiogenesis

Tatsuya Mimura, Jin Hong Chang, Tae Im Kim, Tatsuya Onguchi, Takashi Kojima, Tohru Sakimoto, Dimitri T. Azar

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Abstract

Purpose: Decorin is a small chondroitin sulfate proteoglycan that inhibits vascular endothelial cell migration and tube formation. Membrane type 1ĝ€"matrix metalloproteinase (MT1-MMP) has been shown to be an important angiogenic enzyme in the cornea. We evaluated the specific role of MT1-MMP in decorin cleavage in the cornea. Methods: Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and the stimulatory effect of MT1ĝ€"MMP on vascular endothelial cells in vitro. Corneal micropocket assays using basic fibroblast growth factor (bFGF) were used to assess changes in the levels of decorin and MT1-MMP. Results: MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. MT1-MMP levels were upregulated after in vivo bFGF pellet implantation in the cornea, and decorin cleavage products were detected in bFGF-implanted corneas but not in normal corneas. MT1-MMP reduced the inhibitory effects of decorin on aortic ring tube formation in vitro. CONCLUSION:: MT1-MMP may play an essential role in angiogenesis through proteolytic processing of decorin in the cornea.

Original languageEnglish
Pages (from-to)S45-S49
JournalCornea
Volume30
Issue number10 SUPPL. 1
DOIs
Publication statusPublished - 2011 Oct 1

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All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Mimura, T., Chang, J. H., Kim, T. I., Onguchi, T., Kojima, T., Sakimoto, T., & Azar, D. T. (2011). MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: Role in corneal angiogenesis. Cornea, 30(10 SUPPL. 1), S45-S49. https://doi.org/10.1097/ICO.0b013e31822816e0