MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: Role in corneal angiogenesis

Tatsuya Mimura, Jin Hong Chang, Tae-im Kim, Tatsuya Onguchi, Takashi Kojima, Tohru Sakimoto, Dimitri T. Azar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: Decorin is a small chondroitin sulfate proteoglycan that inhibits vascular endothelial cell migration and tube formation. Membrane type 1ĝ€"matrix metalloproteinase (MT1-MMP) has been shown to be an important angiogenic enzyme in the cornea. We evaluated the specific role of MT1-MMP in decorin cleavage in the cornea. Methods: Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and the stimulatory effect of MT1ĝ€"MMP on vascular endothelial cells in vitro. Corneal micropocket assays using basic fibroblast growth factor (bFGF) were used to assess changes in the levels of decorin and MT1-MMP. Results: MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. MT1-MMP levels were upregulated after in vivo bFGF pellet implantation in the cornea, and decorin cleavage products were detected in bFGF-implanted corneas but not in normal corneas. MT1-MMP reduced the inhibitory effects of decorin on aortic ring tube formation in vitro. CONCLUSION:: MT1-MMP may play an essential role in angiogenesis through proteolytic processing of decorin in the cornea.

Original languageEnglish
JournalCornea
Volume30
Issue number10 SUPPL. 1
DOIs
Publication statusPublished - 2011 Oct 1

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Corneal Neovascularization
Decorin
Matrix Metalloproteinase 14
Proteoglycans
Cornea
Fibroblast Growth Factor 2
Endothelial Cells
Chondroitin Sulfate Proteoglycans
Cell Movement
Western Blotting

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Mimura, T., Chang, J. H., Kim, T., Onguchi, T., Kojima, T., Sakimoto, T., & Azar, D. T. (2011). MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: Role in corneal angiogenesis. Cornea, 30(10 SUPPL. 1). https://doi.org/10.1097/ICO.0b013e31822816e0
Mimura, Tatsuya ; Chang, Jin Hong ; Kim, Tae-im ; Onguchi, Tatsuya ; Kojima, Takashi ; Sakimoto, Tohru ; Azar, Dimitri T. / MT1-MMP cleavage of the antiangiogenic proteoglycan decorin : Role in corneal angiogenesis. In: Cornea. 2011 ; Vol. 30, No. 10 SUPPL. 1.
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Mimura, T, Chang, JH, Kim, T, Onguchi, T, Kojima, T, Sakimoto, T & Azar, DT 2011, 'MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: Role in corneal angiogenesis', Cornea, vol. 30, no. 10 SUPPL. 1. https://doi.org/10.1097/ICO.0b013e31822816e0

MT1-MMP cleavage of the antiangiogenic proteoglycan decorin : Role in corneal angiogenesis. / Mimura, Tatsuya; Chang, Jin Hong; Kim, Tae-im; Onguchi, Tatsuya; Kojima, Takashi; Sakimoto, Tohru; Azar, Dimitri T.

In: Cornea, Vol. 30, No. 10 SUPPL. 1, 01.10.2011.

Research output: Contribution to journalArticle

TY - JOUR

T1 - MT1-MMP cleavage of the antiangiogenic proteoglycan decorin

T2 - Role in corneal angiogenesis

AU - Mimura, Tatsuya

AU - Chang, Jin Hong

AU - Kim, Tae-im

AU - Onguchi, Tatsuya

AU - Kojima, Takashi

AU - Sakimoto, Tohru

AU - Azar, Dimitri T.

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Purpose: Decorin is a small chondroitin sulfate proteoglycan that inhibits vascular endothelial cell migration and tube formation. Membrane type 1ĝ€"matrix metalloproteinase (MT1-MMP) has been shown to be an important angiogenic enzyme in the cornea. We evaluated the specific role of MT1-MMP in decorin cleavage in the cornea. Methods: Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and the stimulatory effect of MT1ĝ€"MMP on vascular endothelial cells in vitro. Corneal micropocket assays using basic fibroblast growth factor (bFGF) were used to assess changes in the levels of decorin and MT1-MMP. Results: MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. MT1-MMP levels were upregulated after in vivo bFGF pellet implantation in the cornea, and decorin cleavage products were detected in bFGF-implanted corneas but not in normal corneas. MT1-MMP reduced the inhibitory effects of decorin on aortic ring tube formation in vitro. CONCLUSION:: MT1-MMP may play an essential role in angiogenesis through proteolytic processing of decorin in the cornea.

AB - Purpose: Decorin is a small chondroitin sulfate proteoglycan that inhibits vascular endothelial cell migration and tube formation. Membrane type 1ĝ€"matrix metalloproteinase (MT1-MMP) has been shown to be an important angiogenic enzyme in the cornea. We evaluated the specific role of MT1-MMP in decorin cleavage in the cornea. Methods: Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and the stimulatory effect of MT1ĝ€"MMP on vascular endothelial cells in vitro. Corneal micropocket assays using basic fibroblast growth factor (bFGF) were used to assess changes in the levels of decorin and MT1-MMP. Results: MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. MT1-MMP levels were upregulated after in vivo bFGF pellet implantation in the cornea, and decorin cleavage products were detected in bFGF-implanted corneas but not in normal corneas. MT1-MMP reduced the inhibitory effects of decorin on aortic ring tube formation in vitro. CONCLUSION:: MT1-MMP may play an essential role in angiogenesis through proteolytic processing of decorin in the cornea.

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