Mtb32 is a promising tuberculosis antigen for DNA vaccination in pre-and post-exposure mouse models

S. S. Ahn, B. Y. Jeon, K. S. Kim, J. Y. Kwack, E. G. Lee, K. S. Park, Y. C. Sung, Sangnae Cho

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Identification of antigens that provide protective immunity via prophylactic and therapeutic vaccination against Mycobacterium tuberculosis is critical for the development of subunit vaccines for tuberculosis (TB). In this study, we performed a head-to-head comparison of seven well-known TB antigens delivered by DNA vaccine, and evaluated their respective immunogenicities and protective efficacies in pre-and post-exposure mouse models. All TB antigens were designed as a chimeric fusion with Flt3-L to enhance antigen-specific T-cell immunity upon vaccination. Prophylactic vaccination with the Flt3L (F)-Mtb32 DNA vaccine elicited significant protection in both the spleen and lungs against M. tuberculosis challenge, comparable to the Bacillus Calmette-Guerin vaccine. F-Ag85A and F-Mtb32 DNA vaccines, in combination with chemotherapy, reduced the bacterial burden to undetectable levels in the lungs of all mice infected with M. tuberculosis. These data collectively indicate that the F-Mtb32 DNA vaccine confers the most efficient protective immunity that suppresses bacterial growth in the active or latent status of M. tuberculosis.

Original languageEnglish
Pages (from-to)570-575
Number of pages6
JournalGene Therapy
Volume19
Issue number5
DOIs
Publication statusPublished - 2012 May 1

Fingerprint

DNA Vaccines
Mycobacterium tuberculosis
Vaccination
Tuberculosis
Antigens
Immunity
DNA
BCG Vaccine
Lung
Subunit Vaccines
Combination Drug Therapy
Spleen
T-Lymphocytes
Growth
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Ahn, S. S. ; Jeon, B. Y. ; Kim, K. S. ; Kwack, J. Y. ; Lee, E. G. ; Park, K. S. ; Sung, Y. C. ; Cho, Sangnae. / Mtb32 is a promising tuberculosis antigen for DNA vaccination in pre-and post-exposure mouse models. In: Gene Therapy. 2012 ; Vol. 19, No. 5. pp. 570-575.
@article{bd22590d3e5943e1884bef25e28a645e,
title = "Mtb32 is a promising tuberculosis antigen for DNA vaccination in pre-and post-exposure mouse models",
abstract = "Identification of antigens that provide protective immunity via prophylactic and therapeutic vaccination against Mycobacterium tuberculosis is critical for the development of subunit vaccines for tuberculosis (TB). In this study, we performed a head-to-head comparison of seven well-known TB antigens delivered by DNA vaccine, and evaluated their respective immunogenicities and protective efficacies in pre-and post-exposure mouse models. All TB antigens were designed as a chimeric fusion with Flt3-L to enhance antigen-specific T-cell immunity upon vaccination. Prophylactic vaccination with the Flt3L (F)-Mtb32 DNA vaccine elicited significant protection in both the spleen and lungs against M. tuberculosis challenge, comparable to the Bacillus Calmette-Guerin vaccine. F-Ag85A and F-Mtb32 DNA vaccines, in combination with chemotherapy, reduced the bacterial burden to undetectable levels in the lungs of all mice infected with M. tuberculosis. These data collectively indicate that the F-Mtb32 DNA vaccine confers the most efficient protective immunity that suppresses bacterial growth in the active or latent status of M. tuberculosis.",
author = "Ahn, {S. S.} and Jeon, {B. Y.} and Kim, {K. S.} and Kwack, {J. Y.} and Lee, {E. G.} and Park, {K. S.} and Sung, {Y. C.} and Sangnae Cho",
year = "2012",
month = "5",
day = "1",
doi = "10.1038/gt.2011.140",
language = "English",
volume = "19",
pages = "570--575",
journal = "Gene Therapy",
issn = "0969-7128",
publisher = "Nature Publishing Group",
number = "5",

}

Ahn, SS, Jeon, BY, Kim, KS, Kwack, JY, Lee, EG, Park, KS, Sung, YC & Cho, S 2012, 'Mtb32 is a promising tuberculosis antigen for DNA vaccination in pre-and post-exposure mouse models', Gene Therapy, vol. 19, no. 5, pp. 570-575. https://doi.org/10.1038/gt.2011.140

Mtb32 is a promising tuberculosis antigen for DNA vaccination in pre-and post-exposure mouse models. / Ahn, S. S.; Jeon, B. Y.; Kim, K. S.; Kwack, J. Y.; Lee, E. G.; Park, K. S.; Sung, Y. C.; Cho, Sangnae.

In: Gene Therapy, Vol. 19, No. 5, 01.05.2012, p. 570-575.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mtb32 is a promising tuberculosis antigen for DNA vaccination in pre-and post-exposure mouse models

AU - Ahn, S. S.

AU - Jeon, B. Y.

AU - Kim, K. S.

AU - Kwack, J. Y.

AU - Lee, E. G.

AU - Park, K. S.

AU - Sung, Y. C.

AU - Cho, Sangnae

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Identification of antigens that provide protective immunity via prophylactic and therapeutic vaccination against Mycobacterium tuberculosis is critical for the development of subunit vaccines for tuberculosis (TB). In this study, we performed a head-to-head comparison of seven well-known TB antigens delivered by DNA vaccine, and evaluated their respective immunogenicities and protective efficacies in pre-and post-exposure mouse models. All TB antigens were designed as a chimeric fusion with Flt3-L to enhance antigen-specific T-cell immunity upon vaccination. Prophylactic vaccination with the Flt3L (F)-Mtb32 DNA vaccine elicited significant protection in both the spleen and lungs against M. tuberculosis challenge, comparable to the Bacillus Calmette-Guerin vaccine. F-Ag85A and F-Mtb32 DNA vaccines, in combination with chemotherapy, reduced the bacterial burden to undetectable levels in the lungs of all mice infected with M. tuberculosis. These data collectively indicate that the F-Mtb32 DNA vaccine confers the most efficient protective immunity that suppresses bacterial growth in the active or latent status of M. tuberculosis.

AB - Identification of antigens that provide protective immunity via prophylactic and therapeutic vaccination against Mycobacterium tuberculosis is critical for the development of subunit vaccines for tuberculosis (TB). In this study, we performed a head-to-head comparison of seven well-known TB antigens delivered by DNA vaccine, and evaluated their respective immunogenicities and protective efficacies in pre-and post-exposure mouse models. All TB antigens were designed as a chimeric fusion with Flt3-L to enhance antigen-specific T-cell immunity upon vaccination. Prophylactic vaccination with the Flt3L (F)-Mtb32 DNA vaccine elicited significant protection in both the spleen and lungs against M. tuberculosis challenge, comparable to the Bacillus Calmette-Guerin vaccine. F-Ag85A and F-Mtb32 DNA vaccines, in combination with chemotherapy, reduced the bacterial burden to undetectable levels in the lungs of all mice infected with M. tuberculosis. These data collectively indicate that the F-Mtb32 DNA vaccine confers the most efficient protective immunity that suppresses bacterial growth in the active or latent status of M. tuberculosis.

UR - http://www.scopus.com/inward/record.url?scp=84862121577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862121577&partnerID=8YFLogxK

U2 - 10.1038/gt.2011.140

DO - 10.1038/gt.2011.140

M3 - Article

VL - 19

SP - 570

EP - 575

JO - Gene Therapy

JF - Gene Therapy

SN - 0969-7128

IS - 5

ER -