Mucosal immunity induced by adenovirus-based H5N1 HPAI vaccine confers protection against a lethal H5N2 avian influenza virus challenge

Ki Seok Park, Jiyeung Lee, So Shin Ahn, Young Ho Byun, Baik Lin Seong, Yun Hee Baek, Min Suk Song, Young Ki Choi, Yun Jeong Na, Inhwan Hwang, Young Chul Sung, Chang Geun Lee

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Development of effective vaccines against highly pathogenic avian influenza (HPAI) H5N1 viruses is a global public health priority. Considering the difficulty in predicting HPAI H5N1 pandemic strains, one strategy used in their design includes the development of formulations with the capacity of eliciting broad cross-protective immunity against multiple viral antigens. To this end we constructed a replication-defective recombinant adenovirus-based avian influenza virus vaccine (rAdv-AI) expressing the codon-optimized M2eX-HA-hCD40L and the M1-M2 fusion genes from HPAI H5N1 human isolate. Although there were no significant differences in the systemic immune responses observed between the intramuscular prime-intramuscular boost regimen (IM/IM) and the intranasal prime-intramuscular boost regimen (IN/IM), IN/IM induced more potent CD8+ T cell and antibody responses at mucosal sites than the IM/IM vaccination, resulting in more effective protection against lethal H5N2 avian influenza (AI) virus challenge. These findings suggest that the strategies used to induce multi-antigen-targeted mucosal immunity, such as IN/IM delivery of rAdv-AI, may be a promising approach for developing broad protective vaccines that may be more effective against the new HPAI pandemic strains.

Original languageEnglish
Pages (from-to)182-189
Number of pages8
JournalVirology
Volume395
Issue number2
DOIs
Publication statusPublished - 2009 Dec 20

Bibliographical note

Funding Information:
We thank Sang-Chun Lee, Kwan Seok Lee, Bok Chae Cho, and Young Jae Choi for devoted animal care and So Young Choi and Yong-bok Seo for technical assistance. This work was supported by grants from Genexine Co. Ltd., Korea and supported by Top Brand Project grant from Korea Research Council of Fundamental Science and Technology and KRIBB Initiative program ( KGM3110912 ).

All Science Journal Classification (ASJC) codes

  • Virology

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