Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes

Jarinyaporn Naowaboot, Choon Hee Chung, Patchareewan Pannangpetch, Ran Choi, Bo Hwan Kim, Mi Young Lee, Upa Kukongviriyapan

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We have previously shown that mulberry leaf extract (MA) causes blood glucose levels to decrease in rats with streptozotocin-induced diabetes while enhancing glucose uptake by isolated fat cells. We hypothesized that the antidiabetic activity of MA is mediated via enhancement of adiponectin secretion and adipogenesis, which consequently decreases blood glucose. In the present study, we aimed to elucidate the molecular basis for the observed antidiabetic activity using murine 3T3-L1 preadipocyte cultures. We found that treatment of differentiating 3T3-L1 cells with MA at concentrations of 5, 15, and 45 μg/mL increased expression of adiponectin messenger RNA from 1.4-fold (control) to 1.5-, 1.95-, and 2.2-fold above basal values, respectively, while causing adiponectin secretion to increase from 70 ± 7.4 ng/mL to 100 ± 1.4, 138 ± 2.0, and 176 ± 21.4 ng/mL, respectively. Furthermore, we observed an increase in both lipid accumulation and messenger RNA expression of transcription factors, such as CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor γ; and of the fatty acid-binding protein aP2 in differentiated 3T3-L1 cells pretreated with MA. Our findings indicate that the stimulatory effects of mulberry leaf extract on adipocyte proliferation and differentiation likely occur through up-regulation of adipogenic transcription factors and downstream gene expression. Such effects of mulberry leaf extract on adiponectin secretion and adipocyte activity may account for, at least in part, the antidiabetic effects of consumption of beverages containing mulberry leaves.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalNutrition Research
Volume32
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Morus
Adiponectin
Adipocytes
Hypoglycemic Agents
3T3-L1 Cells
Blood Glucose
Transcription Factors
CCAAT-Enhancer-Binding Proteins
Fatty Acid-Binding Proteins
Adipogenesis
Messenger RNA
Peroxisome Proliferator-Activated Receptors
Experimental Diabetes Mellitus
Beverages
Up-Regulation
Lipids
Gene Expression
Glucose

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Naowaboot, J., Chung, C. H., Pannangpetch, P., Choi, R., Kim, B. H., Lee, M. Y., & Kukongviriyapan, U. (2012). Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes. Nutrition Research, 32(1), 39-44. https://doi.org/10.1016/j.nutres.2011.12.003
Naowaboot, Jarinyaporn ; Chung, Choon Hee ; Pannangpetch, Patchareewan ; Choi, Ran ; Kim, Bo Hwan ; Lee, Mi Young ; Kukongviriyapan, Upa. / Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes. In: Nutrition Research. 2012 ; Vol. 32, No. 1. pp. 39-44.
@article{65661342d54845ea844021a4b217b12f,
title = "Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes",
abstract = "We have previously shown that mulberry leaf extract (MA) causes blood glucose levels to decrease in rats with streptozotocin-induced diabetes while enhancing glucose uptake by isolated fat cells. We hypothesized that the antidiabetic activity of MA is mediated via enhancement of adiponectin secretion and adipogenesis, which consequently decreases blood glucose. In the present study, we aimed to elucidate the molecular basis for the observed antidiabetic activity using murine 3T3-L1 preadipocyte cultures. We found that treatment of differentiating 3T3-L1 cells with MA at concentrations of 5, 15, and 45 μg/mL increased expression of adiponectin messenger RNA from 1.4-fold (control) to 1.5-, 1.95-, and 2.2-fold above basal values, respectively, while causing adiponectin secretion to increase from 70 ± 7.4 ng/mL to 100 ± 1.4, 138 ± 2.0, and 176 ± 21.4 ng/mL, respectively. Furthermore, we observed an increase in both lipid accumulation and messenger RNA expression of transcription factors, such as CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor γ; and of the fatty acid-binding protein aP2 in differentiated 3T3-L1 cells pretreated with MA. Our findings indicate that the stimulatory effects of mulberry leaf extract on adipocyte proliferation and differentiation likely occur through up-regulation of adipogenic transcription factors and downstream gene expression. Such effects of mulberry leaf extract on adiponectin secretion and adipocyte activity may account for, at least in part, the antidiabetic effects of consumption of beverages containing mulberry leaves.",
author = "Jarinyaporn Naowaboot and Chung, {Choon Hee} and Patchareewan Pannangpetch and Ran Choi and Kim, {Bo Hwan} and Lee, {Mi Young} and Upa Kukongviriyapan",
year = "2012",
month = "1",
day = "1",
doi = "10.1016/j.nutres.2011.12.003",
language = "English",
volume = "32",
pages = "39--44",
journal = "Nutrition Research",
issn = "0271-5317",
publisher = "Elsevier Inc.",
number = "1",

}

Naowaboot, J, Chung, CH, Pannangpetch, P, Choi, R, Kim, BH, Lee, MY & Kukongviriyapan, U 2012, 'Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes', Nutrition Research, vol. 32, no. 1, pp. 39-44. https://doi.org/10.1016/j.nutres.2011.12.003

Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes. / Naowaboot, Jarinyaporn; Chung, Choon Hee; Pannangpetch, Patchareewan; Choi, Ran; Kim, Bo Hwan; Lee, Mi Young; Kukongviriyapan, Upa.

In: Nutrition Research, Vol. 32, No. 1, 01.01.2012, p. 39-44.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes

AU - Naowaboot, Jarinyaporn

AU - Chung, Choon Hee

AU - Pannangpetch, Patchareewan

AU - Choi, Ran

AU - Kim, Bo Hwan

AU - Lee, Mi Young

AU - Kukongviriyapan, Upa

PY - 2012/1/1

Y1 - 2012/1/1

N2 - We have previously shown that mulberry leaf extract (MA) causes blood glucose levels to decrease in rats with streptozotocin-induced diabetes while enhancing glucose uptake by isolated fat cells. We hypothesized that the antidiabetic activity of MA is mediated via enhancement of adiponectin secretion and adipogenesis, which consequently decreases blood glucose. In the present study, we aimed to elucidate the molecular basis for the observed antidiabetic activity using murine 3T3-L1 preadipocyte cultures. We found that treatment of differentiating 3T3-L1 cells with MA at concentrations of 5, 15, and 45 μg/mL increased expression of adiponectin messenger RNA from 1.4-fold (control) to 1.5-, 1.95-, and 2.2-fold above basal values, respectively, while causing adiponectin secretion to increase from 70 ± 7.4 ng/mL to 100 ± 1.4, 138 ± 2.0, and 176 ± 21.4 ng/mL, respectively. Furthermore, we observed an increase in both lipid accumulation and messenger RNA expression of transcription factors, such as CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor γ; and of the fatty acid-binding protein aP2 in differentiated 3T3-L1 cells pretreated with MA. Our findings indicate that the stimulatory effects of mulberry leaf extract on adipocyte proliferation and differentiation likely occur through up-regulation of adipogenic transcription factors and downstream gene expression. Such effects of mulberry leaf extract on adiponectin secretion and adipocyte activity may account for, at least in part, the antidiabetic effects of consumption of beverages containing mulberry leaves.

AB - We have previously shown that mulberry leaf extract (MA) causes blood glucose levels to decrease in rats with streptozotocin-induced diabetes while enhancing glucose uptake by isolated fat cells. We hypothesized that the antidiabetic activity of MA is mediated via enhancement of adiponectin secretion and adipogenesis, which consequently decreases blood glucose. In the present study, we aimed to elucidate the molecular basis for the observed antidiabetic activity using murine 3T3-L1 preadipocyte cultures. We found that treatment of differentiating 3T3-L1 cells with MA at concentrations of 5, 15, and 45 μg/mL increased expression of adiponectin messenger RNA from 1.4-fold (control) to 1.5-, 1.95-, and 2.2-fold above basal values, respectively, while causing adiponectin secretion to increase from 70 ± 7.4 ng/mL to 100 ± 1.4, 138 ± 2.0, and 176 ± 21.4 ng/mL, respectively. Furthermore, we observed an increase in both lipid accumulation and messenger RNA expression of transcription factors, such as CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor γ; and of the fatty acid-binding protein aP2 in differentiated 3T3-L1 cells pretreated with MA. Our findings indicate that the stimulatory effects of mulberry leaf extract on adipocyte proliferation and differentiation likely occur through up-regulation of adipogenic transcription factors and downstream gene expression. Such effects of mulberry leaf extract on adiponectin secretion and adipocyte activity may account for, at least in part, the antidiabetic effects of consumption of beverages containing mulberry leaves.

UR - http://www.scopus.com/inward/record.url?scp=84862946277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862946277&partnerID=8YFLogxK

U2 - 10.1016/j.nutres.2011.12.003

DO - 10.1016/j.nutres.2011.12.003

M3 - Article

VL - 32

SP - 39

EP - 44

JO - Nutrition Research

JF - Nutrition Research

SN - 0271-5317

IS - 1

ER -