Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications

Xia Lou, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a new way of multi-spectral fluorescence microscopy using the embedded liquid filters in a microfluidic chip, providing flexibility of filter changes for target fluorescence wavelengths and low-cost assays for point-of-care (POC) applications. Widely available food color solutions are adopted for emission filter to absorb residual excitation light. Absorbance of the microfluidic liquid filter was measured for various dye concentrations and filter layer thicknesses. Also, absorbance spectrum of two different color mixtures was investigated for tuning the filter wavelength. Both results match with theoretical Beer-Lambert Law. On-chip liquid-filter fluorescence imaging was demonstrated with multiple LED light sources and fluorophores. Live (green)/dead (red) fluorescence images of C6 cells viability assays were successfully demonstrated using the fabricated microchip with the embedded liquid filters. The images taken with the liquid filter were comparable to those from the conventional microscope using FITC HYQ and TRITC HYQ filter sets.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages598-600
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Fluorescence microscopy
Liquids
Fluorescence
Microfluidics
Assays
Color
Wavelength
Fluorophores
Light emitting diodes
Light sources
Microscopes
Dyes
Tuning
Cells
Imaging techniques
Costs

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Lou, X., & Yoon, E. (2011). Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 598-600). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).
Lou, Xia ; Yoon, Euisik. / Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 598-600 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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title = "Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications",
abstract = "We present a new way of multi-spectral fluorescence microscopy using the embedded liquid filters in a microfluidic chip, providing flexibility of filter changes for target fluorescence wavelengths and low-cost assays for point-of-care (POC) applications. Widely available food color solutions are adopted for emission filter to absorb residual excitation light. Absorbance of the microfluidic liquid filter was measured for various dye concentrations and filter layer thicknesses. Also, absorbance spectrum of two different color mixtures was investigated for tuning the filter wavelength. Both results match with theoretical Beer-Lambert Law. On-chip liquid-filter fluorescence imaging was demonstrated with multiple LED light sources and fluorophores. Live (green)/dead (red) fluorescence images of C6 cells viability assays were successfully demonstrated using the fabricated microchip with the embedded liquid filters. The images taken with the liquid filter were comparable to those from the conventional microscope using FITC HYQ and TRITC HYQ filter sets.",
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Lou, X & Yoon, E 2011, Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 1, pp. 598-600, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.

Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications. / Lou, Xia; Yoon, Euisik.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 598-600 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lou X, Yoon E. Multi-spectral fluorescence microscopy with embedded liquid filters for point-of-care applications. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 598-600. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).