Abstract
Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
| Original language | English |
|---|---|
| Pages (from-to) | 12264-12271 |
| Number of pages | 8 |
| Journal | ACS Applied Materials and Interfaces |
| Volume | 9 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2017 Apr 12 |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
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Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells. / Choi, Daheui; Park, Jaeseong; Heo, Jiwoong; Oh, Tong In; Lee, Eunah; Hong, Jinkee.
In: ACS Applied Materials and Interfaces, Vol. 9, No. 14, 12.04.2017, p. 12264-12271.Research output: Contribution to journal › Article
TY - JOUR
T1 - Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells
AU - Choi, Daheui
AU - Park, Jaeseong
AU - Heo, Jiwoong
AU - Oh, Tong In
AU - Lee, Eunah
AU - Hong, Jinkee
PY - 2017/4/12
Y1 - 2017/4/12
N2 - Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
AB - Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
UR - http://www.scopus.com/inward/record.url?scp=85017546420&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017546420&partnerID=8YFLogxK
U2 - 10.1021/acsami.7b00365
DO - 10.1021/acsami.7b00365
M3 - Article
C2 - 28322547
AN - SCOPUS:85017546420
VL - 9
SP - 12264
EP - 12271
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 14
ER -