Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
Bibliographical noteFunding Information:
This research was supported by the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Korean Government (Grant 2012M3A9C6050104 and 2016M3A9C6917405). Additionally, thisresearch was also supported by a grant of the Korea Health Technology R and D Project through the Korea Health Industry Development Institute(KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (Grant HI14C-3266, HI15C-1653). This research was also supported by the Basic Science Research Program (2015R1D1A1A01059702) and Midcareer Research Program (NRF-2015R1A2A2A04006172) through the National Research Foundation of Korea funded by MEST, and by a grant from the Korea Health Technology R and D Project (HI16C1010) through the Korea Health Industry Development Institute (KHIDI) funded by the Korea Ministry of Health and Welfare.
All Science Journal Classification (ASJC) codes
- Materials Science(all)