Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery

Won Gun Koh, Hyun Jong Lee, Hyue Won Lee, Sangphil Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study describes the development of multifunctional micropatterned nanofiber scaffolds consisting of multiple layers of nanofiber. We fabricated double-layered nanofiber scaffolds that were micropatterned with PEG hydrogels by combining two-step sequential electrospinning and hydrogel lithography. We successfully demonstrated that the upper layer could be used to create micropatterns of HepG2 spheroids with uniform size, while the bottom layer could be used for in situ detection of albumin secreted by HepG2 spheroids in the upper layer. Finally, we immobilized growth factors and showed possibility of controlled release of growth factors from micropatterned nanofiber scaffolds.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
Pages83-85
Number of pages3
Publication statusPublished - 2012 Aug 20
EventNanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012 - Santa Clara, CA, United States
Duration: 2012 Jun 182012 Jun 21

Other

OtherNanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
CountryUnited States
CitySanta Clara, CA
Period12/6/1812/6/21

Fingerprint

Nanofibers
Metabolites
Scaffolds
Intercellular Signaling Peptides and Proteins
Hydrogels
Hydrogel
Electrospinning
Lithography
Polyethylene glycols
Albumins

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Surfaces, Coatings and Films

Cite this

Koh, W. G., Lee, H. J., Lee, H. W., & Park, S. (2012). Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery. In Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012 (pp. 83-85)
Koh, Won Gun ; Lee, Hyun Jong ; Lee, Hyue Won ; Park, Sangphil. / Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery. Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012. 2012. pp. 83-85
@inproceedings{f709f5e7035b4d8dba6ec89fa7e85018,
title = "Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery",
abstract = "This study describes the development of multifunctional micropatterned nanofiber scaffolds consisting of multiple layers of nanofiber. We fabricated double-layered nanofiber scaffolds that were micropatterned with PEG hydrogels by combining two-step sequential electrospinning and hydrogel lithography. We successfully demonstrated that the upper layer could be used to create micropatterns of HepG2 spheroids with uniform size, while the bottom layer could be used for in situ detection of albumin secreted by HepG2 spheroids in the upper layer. Finally, we immobilized growth factors and showed possibility of controlled release of growth factors from micropatterned nanofiber scaffolds.",
author = "Koh, {Won Gun} and Lee, {Hyun Jong} and Lee, {Hyue Won} and Sangphil Park",
year = "2012",
month = "8",
day = "20",
language = "English",
isbn = "9781466562769",
pages = "83--85",
booktitle = "Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012",

}

Koh, WG, Lee, HJ, Lee, HW & Park, S 2012, Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery. in Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012. pp. 83-85, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012, Santa Clara, CA, United States, 12/6/18.

Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery. / Koh, Won Gun; Lee, Hyun Jong; Lee, Hyue Won; Park, Sangphil.

Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012. 2012. p. 83-85.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery

AU - Koh, Won Gun

AU - Lee, Hyun Jong

AU - Lee, Hyue Won

AU - Park, Sangphil

PY - 2012/8/20

Y1 - 2012/8/20

N2 - This study describes the development of multifunctional micropatterned nanofiber scaffolds consisting of multiple layers of nanofiber. We fabricated double-layered nanofiber scaffolds that were micropatterned with PEG hydrogels by combining two-step sequential electrospinning and hydrogel lithography. We successfully demonstrated that the upper layer could be used to create micropatterns of HepG2 spheroids with uniform size, while the bottom layer could be used for in situ detection of albumin secreted by HepG2 spheroids in the upper layer. Finally, we immobilized growth factors and showed possibility of controlled release of growth factors from micropatterned nanofiber scaffolds.

AB - This study describes the development of multifunctional micropatterned nanofiber scaffolds consisting of multiple layers of nanofiber. We fabricated double-layered nanofiber scaffolds that were micropatterned with PEG hydrogels by combining two-step sequential electrospinning and hydrogel lithography. We successfully demonstrated that the upper layer could be used to create micropatterns of HepG2 spheroids with uniform size, while the bottom layer could be used for in situ detection of albumin secreted by HepG2 spheroids in the upper layer. Finally, we immobilized growth factors and showed possibility of controlled release of growth factors from micropatterned nanofiber scaffolds.

UR - http://www.scopus.com/inward/record.url?scp=84865025022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865025022&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84865025022

SN - 9781466562769

SP - 83

EP - 85

BT - Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012

ER -

Koh WG, Lee HJ, Lee HW, Park S. Multifunctional micropatterned nanofiber scaffolds capable of cell patterning, metabolite detection, and growth factor delivery. In Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012. 2012. p. 83-85