Multifunctional nanoparticles for gene delivery and spinal cord injury

So Jung Gwak, Heebeom Koo, Yeomin Yun, Ji Young Yhee, Hye Yeong Lee, Do Heum Yoon, Kwangmeyung Kim, Yoon Ha

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI.

Original languageEnglish
Pages (from-to)3474-3482
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number11
DOIs
Publication statusPublished - 2015 Nov 1

Fingerprint

Methylprednisolone
Chitosan
Genes
Nanoparticles
Anti-Inflammatory Agents
Luciferases
Gene therapy
Cell death
Cytotoxicity
Transferases
Labeling
Glucocorticoids
Assays
Plasmids
DNA
Molecular weight
Cells
Apoptosis

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Gwak, So Jung ; Koo, Heebeom ; Yun, Yeomin ; Yhee, Ji Young ; Lee, Hye Yeong ; Yoon, Do Heum ; Kim, Kwangmeyung ; Ha, Yoon. / Multifunctional nanoparticles for gene delivery and spinal cord injury. In: Journal of Biomedical Materials Research - Part A. 2015 ; Vol. 103, No. 11. pp. 3474-3482.
@article{4f0c8068b74b423091c0ab519aeababf,
title = "Multifunctional nanoparticles for gene delivery and spinal cord injury",
abstract = "Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI.",
author = "Gwak, {So Jung} and Heebeom Koo and Yeomin Yun and Yhee, {Ji Young} and Lee, {Hye Yeong} and Yoon, {Do Heum} and Kwangmeyung Kim and Yoon Ha",
year = "2015",
month = "11",
day = "1",
doi = "10.1002/jbm.a.35489",
language = "English",
volume = "103",
pages = "3474--3482",
journal = "Journal of Biomedical Materials Research",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

Multifunctional nanoparticles for gene delivery and spinal cord injury. / Gwak, So Jung; Koo, Heebeom; Yun, Yeomin; Yhee, Ji Young; Lee, Hye Yeong; Yoon, Do Heum; Kim, Kwangmeyung; Ha, Yoon.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 11, 01.11.2015, p. 3474-3482.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multifunctional nanoparticles for gene delivery and spinal cord injury

AU - Gwak, So Jung

AU - Koo, Heebeom

AU - Yun, Yeomin

AU - Yhee, Ji Young

AU - Lee, Hye Yeong

AU - Yoon, Do Heum

AU - Kim, Kwangmeyung

AU - Ha, Yoon

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI.

AB - Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI.

UR - http://www.scopus.com/inward/record.url?scp=84942836354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942836354&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.35489

DO - 10.1002/jbm.a.35489

M3 - Article

VL - 103

SP - 3474

EP - 3482

JO - Journal of Biomedical Materials Research

JF - Journal of Biomedical Materials Research

SN - 1549-3296

IS - 11

ER -