Biologically versatile basic fibroblast growth factor (bFGF), well known for roles of signaling molecules between cells and regulating various cellular processes, has been proven to utilize specific functionalities. However, the remarkable functions are inclinable to dwindle with decrease of bFGFs' activity. In nanoscale, developing thin films with intrinsic characteristics of building molecules can facilitate handling various materials for desired purposes. Fabricating nanofilm and handling sensitive materials without detriment to activity via highly productive manufacturing are significant for practical uses in the field of biomedical applications. Herein, a multilayered nanofilm fabricating system is developed by inkjet printing to incorporate bFGF successfully. It is demonstrated that water mixed with glycerol as biological ink maintains stability of bFGFs through simulation and experimental study. With highly stable bFGFs, the proliferation of human dermal fibroblast is enhanced and the undifferentiated state of induced pluripotent stem cell is maintained by the controlled release of bFGF.
Bibliographical noteFunding Information:
M.C. and H.H.P. contributed equally to this work. This research was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning of Korea government (Grant Nos. 2012M3A9C6050100, 2012M3A9C6050104, 2016M3A9C6917405, and 2015R1C1A1A01052831). Additionally, this research was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant Nos. HI14C-3266 and HI15C-1653).
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Pharmaceutical Science