Multimodal biophotonic workstation for live cell analysis

Michael Esseling, Björn Kemper, Maciej Antkowiak, David J. Stevenson, Lionel Chaudet, Mark A.A. Neil, Paul W. French, Gert von Bally, Kishan Dholakia, Cornelia Denz

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Abstract

A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalJournal of Biophotonics
Volume5
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Esseling, M., Kemper, B., Antkowiak, M., Stevenson, D. J., Chaudet, L., Neil, M. A. A., French, P. W., von Bally, G., Dholakia, K., & Denz, C. (2012). Multimodal biophotonic workstation for live cell analysis. Journal of Biophotonics, 5(1), 9-13. https://doi.org/10.1002/jbio.201100052