Multimodal biophotonic workstation for live cell analysis

Michael Esseling; Björn Kemper; Maciej Antkowiak; David J. Stevenson; Lionel Chaudet; Mark A.A. Neil; Paul W. French; Gert von Bally; Kishan Dholakia; Cornelia Denz

doi:10.1002/jbio.201100052

Multimodal biophotonic workstation for live cell analysis

Michael Esseling, Björn Kemper, Maciej Antkowiak, David J. Stevenson, Lionel Chaudet, Mark A.A. Neil, Paul W. French, Gert von Bally, Kishan Dholakia, Cornelia Denz

Department of Physics

Research output: Contribution to journal › Letter › peer-review

22 Citations (Scopus)

Abstract

A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.

Original language	English
Pages (from-to)	9-13
Number of pages	5
Journal	Journal of Biophotonics
Volume	5
Issue number	1
DOIs	https://doi.org/10.1002/jbio.201100052
Publication status	Published - 2012 Jan

All Science Journal Classification (ASJC) codes

Chemistry(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)
Physics and Astronomy(all)

Access to Document

10.1002/jbio.201100052

Fingerprint Dive into the research topics of 'Multimodal biophotonic workstation for live cell analysis'. Together they form a unique fingerprint.

Cite this

@article{9b28abb3a6314f4b98ac8a2e6fcb10c1,

title = "Multimodal biophotonic workstation for live cell analysis",

abstract = "A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.",

author = "Michael Esseling and Bj{\"o}rn Kemper and Maciej Antkowiak and Stevenson, {David J.} and Lionel Chaudet and Neil, {Mark A.A.} and French, {Paul W.} and {von Bally}, Gert and Kishan Dholakia and Cornelia Denz",

year = "2012",

month = jan,

doi = "10.1002/jbio.201100052",

language = "English",

volume = "5",

pages = "9--13",

journal = "Journal of Biophotonics",

issn = "1864-063X",

publisher = "Wiley-VCH Verlag",

number = "1",

}

TY - JOUR

T1 - Multimodal biophotonic workstation for live cell analysis

AU - Esseling, Michael

AU - Kemper, Björn

AU - Antkowiak, Maciej

AU - Stevenson, David J.

AU - Chaudet, Lionel

AU - Neil, Mark A.A.

AU - French, Paul W.

AU - von Bally, Gert

AU - Dholakia, Kishan

AU - Denz, Cornelia

PY - 2012/1

Y1 - 2012/1

N2 - A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.

AB - A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.

UR - http://www.scopus.com/inward/record.url?scp=83155190280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83155190280&partnerID=8YFLogxK

U2 - 10.1002/jbio.201100052

DO - 10.1002/jbio.201100052

M3 - Letter

C2 - 21842486

AN - SCOPUS:83155190280

VL - 5

SP - 9

EP - 13

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 1

ER -