Multimodal biophotonic workstation for live cell analysis

Michael Esseling; Björn Kemper; Maciej Antkowiak; David J. Stevenson; Lionel Chaudet; Mark A.A. Neil; Paul W. French; Gert von Bally; Kishan Dholakia; Cornelia Denz

doi:10.1002/jbio.201100052

Multimodal biophotonic workstation for live cell analysis

Michael Esseling, Björn Kemper, Maciej Antkowiak, David J. Stevenson, Lionel Chaudet, Mark A.A. Neil, Paul W. French, Gert von Bally, Kishan Dholakia, Cornelia Denz

Department of Physics

Research output: Contribution to journal › Letter › peer-review

23 Citations (Scopus)

Abstract

A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.

Original language	English
Pages (from-to)	9-13
Number of pages	5
Journal	Journal of Biophotonics
Volume	5
Issue number	1
DOIs	https://doi.org/10.1002/jbio.201100052
Publication status	Published - 2012 Jan

All Science Journal Classification (ASJC) codes

Chemistry(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)
Physics and Astronomy(all)

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10.1002/jbio.201100052

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abstract = "A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.",

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AU - Esseling, Michael

AU - Kemper, Björn

AU - Antkowiak, Maciej

AU - Stevenson, David J.

AU - Chaudet, Lionel

AU - Neil, Mark A.A.

AU - French, Paul W.

AU - von Bally, Gert

AU - Dholakia, Kishan

AU - Denz, Cornelia

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AB - A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.

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Multimodal biophotonic workstation for live cell analysis

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All Science Journal Classification (ASJC) codes

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