Multimodal in vivo MRI and NIRF imaging of bladder tumor using peptide conjugated glycol chitosan nanoparticles

Jaehong Key, Deepika Dhawan, Deborah W. Knapp, Kwangmeyung Kim, Ick Chan Kwon, Kuiwon Choi, James F. Leary

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Exact detection and complete removal of cancer is a key point to minimize cancer recurrence. However, it is currently very difficult to detect small tumors inside human body and continuously monitor tumors using a non-invasive imaging modality. Presently, positron emission tomography (PET) can provide the most sensitive cancer images in the human body. However, PET imaging has very limited imaging time because they typically use isotopes with short halflives. PET imaging cannot also visualize anatomical information. Magnetic resonance imaging (MRI) can provide highresolution images inside the body but it has a low sensitivity, so MRI contrast agents are necessary to enhance the contrast of tumor. Near infrared fluorescent (NIRF) imaging has a good sensitivity to visualize tumor using optical probes, but it has a very limited tissue penetration depth. Therefore, we developed multi-modality nanoparticles for MRI based diagnosis and NIRF imaging based surgery of cancer. We utilized glycol chitosan of 350 nm as a vehicle for MRI contrast agents and NIRF probes. The glycol chitosan nanoparticles were conjugated with NIRF dye, Cy5.5 and bladder cancer targeting peptides to increase the internalization of cancer. For MR contrast effects, iron oxide based 22 nm nanocubes were physically loaded into the glycol chitosan nanoparticles. The nanoparticles were characterized and evaluated in bladder tumor bearing mice. Our study suggests the potential of our nanoparticles by both MRI and NIRF imaging for tumor diagnosis and real-time NIRF image-guided tumor surgery.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
DOIs
Publication statusPublished - 2012 Apr 2
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X - San Francisco, CA, United States
Duration: 2012 Jan 212012 Jan 23

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8225
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
CountryUnited States
CitySan Francisco, CA
Period12/1/2112/1/23

Fingerprint

bladder
Glycols
Chitosan
Urinary Bladder Neoplasms
Nanoparticles
Magnetic resonance imaging
Peptides
peptides
magnetic resonance
glycols
Tumors
tumors
Magnetic Resonance Imaging
Infrared radiation
Imaging techniques
cancer
nanoparticles
Positron emission tomography
Neoplasms
positrons

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Key, J., Dhawan, D., Knapp, D. W., Kim, K., Kwon, I. C., Choi, K., & Leary, J. F. (2012). Multimodal in vivo MRI and NIRF imaging of bladder tumor using peptide conjugated glycol chitosan nanoparticles. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X [82251F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8225). https://doi.org/10.1117/12.908901
Key, Jaehong ; Dhawan, Deepika ; Knapp, Deborah W. ; Kim, Kwangmeyung ; Kwon, Ick Chan ; Choi, Kuiwon ; Leary, James F. / Multimodal in vivo MRI and NIRF imaging of bladder tumor using peptide conjugated glycol chitosan nanoparticles. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X. 2012. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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abstract = "Exact detection and complete removal of cancer is a key point to minimize cancer recurrence. However, it is currently very difficult to detect small tumors inside human body and continuously monitor tumors using a non-invasive imaging modality. Presently, positron emission tomography (PET) can provide the most sensitive cancer images in the human body. However, PET imaging has very limited imaging time because they typically use isotopes with short halflives. PET imaging cannot also visualize anatomical information. Magnetic resonance imaging (MRI) can provide highresolution images inside the body but it has a low sensitivity, so MRI contrast agents are necessary to enhance the contrast of tumor. Near infrared fluorescent (NIRF) imaging has a good sensitivity to visualize tumor using optical probes, but it has a very limited tissue penetration depth. Therefore, we developed multi-modality nanoparticles for MRI based diagnosis and NIRF imaging based surgery of cancer. We utilized glycol chitosan of 350 nm as a vehicle for MRI contrast agents and NIRF probes. The glycol chitosan nanoparticles were conjugated with NIRF dye, Cy5.5 and bladder cancer targeting peptides to increase the internalization of cancer. For MR contrast effects, iron oxide based 22 nm nanocubes were physically loaded into the glycol chitosan nanoparticles. The nanoparticles were characterized and evaluated in bladder tumor bearing mice. Our study suggests the potential of our nanoparticles by both MRI and NIRF imaging for tumor diagnosis and real-time NIRF image-guided tumor surgery.",
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Key, J, Dhawan, D, Knapp, DW, Kim, K, Kwon, IC, Choi, K & Leary, JF 2012, Multimodal in vivo MRI and NIRF imaging of bladder tumor using peptide conjugated glycol chitosan nanoparticles. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X., 82251F, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, San Francisco, CA, United States, 12/1/21. https://doi.org/10.1117/12.908901

Multimodal in vivo MRI and NIRF imaging of bladder tumor using peptide conjugated glycol chitosan nanoparticles. / Key, Jaehong; Dhawan, Deepika; Knapp, Deborah W.; Kim, Kwangmeyung; Kwon, Ick Chan; Choi, Kuiwon; Leary, James F.

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X. 2012. 82251F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8225).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Key J, Dhawan D, Knapp DW, Kim K, Kwon IC, Choi K et al. Multimodal in vivo MRI and NIRF imaging of bladder tumor using peptide conjugated glycol chitosan nanoparticles. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X. 2012. 82251F. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.908901