Nonlinear optical microscopy has enabled the possibility to explore inside the living organisms. It utilizes ultrashort laser pulse with long wavelength (greater than 800nm). Ultrashort pulse produces high peak power to induce nonlinear optical phenomenon such as two-photon excitation fluorescence (TPEF) and harmonic generations in the medium while maintaining relatively low average energy pre area. In plant developmental biology, confocal microscopy is widely used in plant cell imaging after the development of biological fluorescence labels in mid-1990s. However, fluorescence labeling itself affects the sample and the sample deviates from intact condition especially when labelling the entire cell. In this work, we report the dynamic images of Arabidopsis thaliana root cells. This demonstrates the multimodal nonlinear optical microscopy is an effective tool for long-term plant cell imaging.
|Title of host publication||International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017|
|Editors||Jaebum Choo, Seung-Han Park|
|Publication status||Published - 2017|
|Event||International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 - Jeju, Korea, Republic of|
Duration: 2017 Feb 22 → 2017 Feb 24
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Other||International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017|
|Country||Korea, Republic of|
|Period||17/2/22 → 17/2/24|
Bibliographical notePublisher Copyright:
© 2017 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging