Multimodal nonlinear imaging of arabidopsis thaliana root cell

Bumjoon Jang, Sung Ho Lee, Sooah Woo, Jong Hyun Park, Myeong Min Lee, Seung Han Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nonlinear optical microscopy has enabled the possibility to explore inside the living organisms. It utilizes ultrashort laser pulse with long wavelength (greater than 800nm). Ultrashort pulse produces high peak power to induce nonlinear optical phenomenon such as two-photon excitation fluorescence (TPEF) and harmonic generations in the medium while maintaining relatively low average energy pre area. In plant developmental biology, confocal microscopy is widely used in plant cell imaging after the development of biological fluorescence labels in mid-1990s. However, fluorescence labeling itself affects the sample and the sample deviates from intact condition especially when labelling the entire cell. In this work, we report the dynamic images of Arabidopsis thaliana root cells. This demonstrates the multimodal nonlinear optical microscopy is an effective tool for long-term plant cell imaging.

Original languageEnglish
Title of host publicationInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
EditorsJaebum Choo, Seung-Han Park
PublisherSPIE
ISBN (Electronic)9781510610934
DOIs
Publication statusPublished - 2017 Jan 1
EventInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 - Jeju, Korea, Republic of
Duration: 2017 Feb 222017 Feb 24

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10324
ISSN (Print)1605-7422

Other

OtherInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
CountryKorea, Republic of
CityJeju
Period17/2/2217/2/24

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Jang, B., Lee, S. H., Woo, S., Park, J. H., Lee, M. M., & Park, S. H. (2017). Multimodal nonlinear imaging of arabidopsis thaliana root cell. In J. Choo, & S-H. Park (Eds.), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 [103240C] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10324). SPIE. https://doi.org/10.1117/12.2270697