Multimodal nonlinear microscopy at 1.5 μm

Chun Zhan, Chulmin Joo, Qing Li, Mikhail Y. Berezin, Walter J. Akers, Yunpeng Ye, Samuel Achilefu, Siavash Yazdanfar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences X
DOIs
Publication statusPublished - 2010 May 7
EventMultiphoton Microscopy in the Biomedical Sciences X - San Francisco, CA, United States
Duration: 2010 Jan 242010 Jan 26

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7569
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences X
CountryUnited States
CitySan Francisco, CA
Period10/1/2410/1/26

Fingerprint

Microscopy
Microscopic examination
microscopy
Imaging techniques
Confocal microscopy
Fluorescence microscopy
Fiber lasers
Photons
Fluorescence Microscopy
Confocal Microscopy
Contrast Media
Lasers
Infrared radiation
excitation
fiber lasers
emission spectra
penetration
platforms
fluorescence
photons

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Zhan, C., Joo, C., Li, Q., Berezin, M. Y., Akers, W. J., Ye, Y., ... Yazdanfar, S. (2010). Multimodal nonlinear microscopy at 1.5 μm. In Multiphoton Microscopy in the Biomedical Sciences X [75691Z] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7569). https://doi.org/10.1117/12.842573
Zhan, Chun ; Joo, Chulmin ; Li, Qing ; Berezin, Mikhail Y. ; Akers, Walter J. ; Ye, Yunpeng ; Achilefu, Samuel ; Yazdanfar, Siavash. / Multimodal nonlinear microscopy at 1.5 μm. Multiphoton Microscopy in the Biomedical Sciences X. 2010. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Zhan, C, Joo, C, Li, Q, Berezin, MY, Akers, WJ, Ye, Y, Achilefu, S & Yazdanfar, S 2010, Multimodal nonlinear microscopy at 1.5 μm. in Multiphoton Microscopy in the Biomedical Sciences X., 75691Z, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7569, Multiphoton Microscopy in the Biomedical Sciences X, San Francisco, CA, United States, 10/1/24. https://doi.org/10.1117/12.842573

Multimodal nonlinear microscopy at 1.5 μm. / Zhan, Chun; Joo, Chulmin; Li, Qing; Berezin, Mikhail Y.; Akers, Walter J.; Ye, Yunpeng; Achilefu, Samuel; Yazdanfar, Siavash.

Multiphoton Microscopy in the Biomedical Sciences X. 2010. 75691Z (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7569).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Zhan C, Joo C, Li Q, Berezin MY, Akers WJ, Ye Y et al. Multimodal nonlinear microscopy at 1.5 μm. In Multiphoton Microscopy in the Biomedical Sciences X. 2010. 75691Z. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.842573