Optical clearing is emerging as a popular approach particularly for studies in neuroscience. However the use of corrosive clearing solutions typically requires sophisticated objectives or extreme care with optical components chosen for single- or multi-photon imaging. In contrast to the use of complex, custom-made microscope objectives, we show that the use of a corrected multimode fibre (MMF) offers a route that is resistant to corrosion, can be used in clearing media, is not constrained by the refractive index of the immersion medium and offers flexible working distances. Using a corrected MMF, we demonstrate fluorescence imaging of beads and stained neuroblastoma cells through optically cleared mouse brain tissue, as well as imaging in an extreme oxidative environment to show the versatility of our approach. Additionally, we perform Raman imaging of polystyrene beads in clearing media to demonstrate that this approach may be used for vibrational spectroscopy of optically cleared samples.
Bibliographical noteFunding Information:
IG acknowledges support of a Marie Skłodowska-Curie Action Fellowship, as this project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 707084. JAT acknowledges support from the British Society for Neuroendocrinology and the Northwood Charitable Trust. KD acknowledges support of a UK EPSRC Programme Grant (EP/P030017/1). We thank Frances E. Goff for providing and culturing the SH-SY5Y cell line, as well as Blair Kirkpatrick and Dr. Pavlos Manousiadis for their assistance in SEM imaging of the fibres.
UK Engineering and Physical Sciences Research Council (EPSRC) (EP/P030017/1); European Union’s Horizon 2020 Marie Skłodowska-Curie Actions (MSCA) (707084).
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics