Multimode fibre: Light-sheet microscopy at the tip of a needle

Martin Plöschner, Věra Kollárová, Zbyněk Dostál, Jonathan Nylk, Thomas Barton-Owen, David E.K. Ferrier, Radim Chmelík, Kishan Dholakia, Tomáš Čižmár

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre - an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Original languageEnglish
Article number18050
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 14

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Needles
Microscopy
Light
Holography
Optical Fibers
Three-Dimensional Imaging
Biological Science Disciplines
Lighting
Fluorescence Microscopy
Sepharose
Gels

All Science Journal Classification (ASJC) codes

  • General

Cite this

Plöschner, M., Kollárová, V., Dostál, Z., Nylk, J., Barton-Owen, T., Ferrier, D. E. K., ... Čižmár, T. (2015). Multimode fibre: Light-sheet microscopy at the tip of a needle. Scientific reports, 5, [18050]. https://doi.org/10.1038/srep18050
Plöschner, Martin ; Kollárová, Věra ; Dostál, Zbyněk ; Nylk, Jonathan ; Barton-Owen, Thomas ; Ferrier, David E.K. ; Chmelík, Radim ; Dholakia, Kishan ; Čižmár, Tomáš. / Multimode fibre : Light-sheet microscopy at the tip of a needle. In: Scientific reports. 2015 ; Vol. 5.
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Plöschner, M, Kollárová, V, Dostál, Z, Nylk, J, Barton-Owen, T, Ferrier, DEK, Chmelík, R, Dholakia, K & Čižmár, T 2015, 'Multimode fibre: Light-sheet microscopy at the tip of a needle', Scientific reports, vol. 5, 18050. https://doi.org/10.1038/srep18050

Multimode fibre : Light-sheet microscopy at the tip of a needle. / Plöschner, Martin; Kollárová, Věra; Dostál, Zbyněk; Nylk, Jonathan; Barton-Owen, Thomas; Ferrier, David E.K.; Chmelík, Radim; Dholakia, Kishan; Čižmár, Tomáš.

In: Scientific reports, Vol. 5, 18050, 14.12.2015.

Research output: Contribution to journalArticle

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AU - Nylk, Jonathan

AU - Barton-Owen, Thomas

AU - Ferrier, David E.K.

AU - Chmelík, Radim

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AU - Čižmár, Tomáš

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Plöschner M, Kollárová V, Dostál Z, Nylk J, Barton-Owen T, Ferrier DEK et al. Multimode fibre: Light-sheet microscopy at the tip of a needle. Scientific reports. 2015 Dec 14;5. 18050. https://doi.org/10.1038/srep18050