Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds

Min Jeong Kim, Yong Cheol Shin, Jong Ho Lee, Seung Won Jun, Chang Seok Kim, Yunki Lee, Jongchul Park, Soo Hong Lee, Ki Dong Park, Dong Wook Han

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Hydrogels can serve as three-dimensional (3D) scaffolds for cell culture and be readily injected into the body. Recent advances in the image technology for 3D scaffolds like hydrogels have attracted considerable attention to overcome the drawbacks of ordinary imaging technologies such as optical and fluorescence microscopy. Multiphoton microscopy (MPM) is an effective method based on the excitation of two-photons. In the present study, C2C12 myoblasts differentiated in 3D gelatin hydroxyphenylpropionic acid (GHPA) hydrogels were imaged by using a custom-built multiphoton excitation fluorescence microscopy to compare the difference in the imaging capacity between conventional microscopy and MPM. Results: The physicochemical properties of GHPA hydrogels were characterized by using scanning electron microscopy and Fourier-transform infrared spectroscopy. In addition, the cell viability and proliferation of C2C12 myoblasts cultured in the GHPA hydrogels were analyzed by using Live/Dead Cell and CCK-8 assays, respectively. It was found that C2C12 cells were well grown and normally proliferated in the hydrogels. Furthermore, the hydrogels were shown to be suitable to facilitate the myogenic differentiation of C2C12 cells incubated in differentiation media, which had been corroborated by MPM. It was very hard to get clear images from a fluorescence microscope. Conclusions: Our findings suggest that the gelatin-based hydrogels can be beneficially utilized as 3D scaffolds for skeletal muscle engineering and that MPM can be effectively applied to imaging technology for tissue regeneration.

Original languageEnglish
Article number2
JournalBiomaterials Research
Volume20
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Tissue Scaffolds
Hydrogels
Gelatin
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Imaging techniques
Microscopy
Microscopic examination
Myoblasts
Fluorescence microscopy
Technology
Scaffolds
Multiphoton Fluorescence Microscopy
Acids
Sincalide
Tissue regeneration
Fourier Transform Infrared Spectroscopy
Photons
Fluorescence Microscopy

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Kim, Min Jeong ; Shin, Yong Cheol ; Lee, Jong Ho ; Jun, Seung Won ; Kim, Chang Seok ; Lee, Yunki ; Park, Jongchul ; Lee, Soo Hong ; Park, Ki Dong ; Han, Dong Wook. / Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds. In: Biomaterials Research. 2016 ; Vol. 20, No. 1.
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Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds. / Kim, Min Jeong; Shin, Yong Cheol; Lee, Jong Ho; Jun, Seung Won; Kim, Chang Seok; Lee, Yunki; Park, Jongchul; Lee, Soo Hong; Park, Ki Dong; Han, Dong Wook.

In: Biomaterials Research, Vol. 20, No. 1, 2, 01.01.2016.

Research output: Contribution to journalArticle

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