Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y. Berezin, Walter J. Akers, Samuel Achilefu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original languageEnglish
Article number030505
JournalJournal of Biomedical Optics
Volume15
Issue number3
DOIs
Publication statusPublished - 2010 May 1

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Contrast Media
Microscopic examination
microscopy
Infrared radiation
Molecular Probes
Ultrafast lasers
Fluorophores
visible spectrum
excitation
Telecommunication
telecommunication
Fluorescence
Wavelength
fluorescence
fibers
Fibers
probes
wavelengths
lasers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Yazdanfar, S., Joo, C., Zhan, C., Berezin, M. Y., Akers, W. J., & Achilefu, S. (2010). Multiphoton microscopy with near infrared contrast agents. Journal of Biomedical Optics, 15(3), [030505]. https://doi.org/10.1117/1.3420209
Yazdanfar, Siavash ; Joo, Chulmin ; Zhan, Chun ; Berezin, Mikhail Y. ; Akers, Walter J. ; Achilefu, Samuel. / Multiphoton microscopy with near infrared contrast agents. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 3.
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Yazdanfar, S, Joo, C, Zhan, C, Berezin, MY, Akers, WJ & Achilefu, S 2010, 'Multiphoton microscopy with near infrared contrast agents', Journal of Biomedical Optics, vol. 15, no. 3, 030505. https://doi.org/10.1117/1.3420209

Multiphoton microscopy with near infrared contrast agents. / Yazdanfar, Siavash; Joo, Chulmin; Zhan, Chun; Berezin, Mikhail Y.; Akers, Walter J.; Achilefu, Samuel.

In: Journal of Biomedical Optics, Vol. 15, No. 3, 030505, 01.05.2010.

Research output: Contribution to journalArticle

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Yazdanfar S, Joo C, Zhan C, Berezin MY, Akers WJ, Achilefu S. Multiphoton microscopy with near infrared contrast agents. Journal of Biomedical Optics. 2010 May 1;15(3). 030505. https://doi.org/10.1117/1.3420209