Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y. Berezin, Walter J. Akers, Samuel Achilefu

Research output: Contribution to journalArticle

27 Citations (Scopus)


While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original languageEnglish
Article number030505
JournalJournal of Biomedical Optics
Issue number3
Publication statusPublished - 2010 May 1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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    Yazdanfar, S., Joo, C., Zhan, C., Berezin, M. Y., Akers, W. J., & Achilefu, S. (2010). Multiphoton microscopy with near infrared contrast agents. Journal of Biomedical Optics, 15(3), [030505].