Multiple airborne allergen-induced eosinophilic chronic rhinosinusitis murine model

Sang Chul Park, Soo In Kim, Chi Sang Hwang, Hyung Ju Cho, Joo Heon Yoon, Chang-Hoon Kim

Research output: Contribution to journalArticle

Abstract

Purpose: Several murine models have been established to mimic human eosinophilic chronic rhinosinusitis (ECRS). However, in most of these models, ECRS was induced using ovalbumin, which does not cause sinusitis in humans. Thus, we aimed to develop a more clinically relevant murine model of ECRS using multiple airborne allergens. We also investigated the effects of exposure duration of the allergens on ECRS development. Methods: C57BL/6 mice were intranasally administered multiple airborne allergens (house dust mite, Aspergillus fumigatus, Alternaria alternata, and protease from Staphylococcus aureus) three times weekly for 4, 8, 12, and 16 consecutive weeks. Histopathological changes, the levels of cytokines and chemokines in the nasal lavage fluid, and immune cells of the blood and spleen were analyzed. Results: The mice administered multiple allergens showed significantly increased eosinophil infiltration, epithelial thickening and disruption, and subepithelial collagen deposition from 8 weeks compared to the control group. Goblet cell hyperplasia, polyp-like lesions, and blood eosinophils, as well as the levels of interleukin-5 and eotaxin in the nasal lavage fluid were considerably increased in the ECRS group from 12 weeks compared to those of controls. Instillation of allergens for 16 weeks exacerbated the eosinophil infiltration and eotaxin increase in the nasal lavage fluid. Conclusions: We successfully established a new murine model of ECRS using more clinically relevant multiple airborne allergens. Prolonged exposure to airborne allergens for 12 weeks or more, corresponding to the definition of human ECRS, strongly induced eosinophil infiltration as well as epithelial remodeling.

Original languageEnglish
Pages (from-to)2273-2282
Number of pages10
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume276
Issue number8
DOIs
Publication statusPublished - 2019 Aug 1

Fingerprint

Allergens
Nasal Lavage Fluid
Eosinophils
Dermatophagoides Antigens
Alternaria
Aspergillus fumigatus
Goblet Cells
Sinusitis
Interleukin-5
Ovalbumin
Polyps
Inbred C57BL Mouse
Chemokines
Hyperplasia
Staphylococcus aureus
Blood Cells
Peptide Hydrolases
Collagen
Spleen
Cytokines

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Park, Sang Chul ; Kim, Soo In ; Hwang, Chi Sang ; Cho, Hyung Ju ; Yoon, Joo Heon ; Kim, Chang-Hoon. / Multiple airborne allergen-induced eosinophilic chronic rhinosinusitis murine model. In: European Archives of Oto-Rhino-Laryngology. 2019 ; Vol. 276, No. 8. pp. 2273-2282.
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Multiple airborne allergen-induced eosinophilic chronic rhinosinusitis murine model. / Park, Sang Chul; Kim, Soo In; Hwang, Chi Sang; Cho, Hyung Ju; Yoon, Joo Heon; Kim, Chang-Hoon.

In: European Archives of Oto-Rhino-Laryngology, Vol. 276, No. 8, 01.08.2019, p. 2273-2282.

Research output: Contribution to journalArticle

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AU - Cho, Hyung Ju

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AU - Kim, Chang-Hoon

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