Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice

Implication for posterior lenticonus with cataract

Seung Whan Kim, Cheolho Cheong, Young Chang Sohn, Young Hwa Goo, Wan Je Oh, Jung Hwan Park, So Young Joe, Hyen Sam Kang, Duk Kyung Kim, Changwon Kee, Jae Woon Lee, Han Woong Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

ASC-2, a recently isolated transcriptional coactivator molecule, stimulates transactivation by multiple transcription factors, including nuclear receptors. We generated a potent dominant negative fragment of ASC-2, encompassing the N-terminal LXXLL motif that binds a broad range of nuclear receptors. This fragment, termed DN1, specifically inhibited endogenous ASC-2 from binding these receptors in vivo, whereas DN1/m, in which the LXXLL motif was mutated to LXXAA to abolish the receptor interactions, was inert. Interestingly, DN1 transgenic mice but not DN1/m transgenic mice exhibited severe microphthalmia and posterior lenticonus with cataract as well as a variety of pathophysiological phenotypes in many other organs. Our results provide a novel insight into the molecular and histopathological mechanism of posterior lenticonus with cataract and attest to the importance of ASC-2 as a pivotal transcriptional coactivator of nuclear receptors in vivo.

Original languageEnglish
Pages (from-to)8409-8414
Number of pages6
JournalMolecular and Cellular Biology
Volume22
Issue number24
DOIs
Publication statusPublished - 2002 Dec 1

Fingerprint

Cytoplasmic and Nuclear Receptors
Cataract
Transgenic Mice
Nuclear Receptor Coactivators
Microphthalmos
Transcriptional Activation
Transcription Factors
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Kim, Seung Whan ; Cheong, Cheolho ; Sohn, Young Chang ; Goo, Young Hwa ; Oh, Wan Je ; Park, Jung Hwan ; Joe, So Young ; Kang, Hyen Sam ; Kim, Duk Kyung ; Kee, Changwon ; Lee, Jae Woon ; Lee, Han Woong. / Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice : Implication for posterior lenticonus with cataract. In: Molecular and Cellular Biology. 2002 ; Vol. 22, No. 24. pp. 8409-8414.
@article{e51f19ad71704348825c744011582bdc,
title = "Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice: Implication for posterior lenticonus with cataract",
abstract = "ASC-2, a recently isolated transcriptional coactivator molecule, stimulates transactivation by multiple transcription factors, including nuclear receptors. We generated a potent dominant negative fragment of ASC-2, encompassing the N-terminal LXXLL motif that binds a broad range of nuclear receptors. This fragment, termed DN1, specifically inhibited endogenous ASC-2 from binding these receptors in vivo, whereas DN1/m, in which the LXXLL motif was mutated to LXXAA to abolish the receptor interactions, was inert. Interestingly, DN1 transgenic mice but not DN1/m transgenic mice exhibited severe microphthalmia and posterior lenticonus with cataract as well as a variety of pathophysiological phenotypes in many other organs. Our results provide a novel insight into the molecular and histopathological mechanism of posterior lenticonus with cataract and attest to the importance of ASC-2 as a pivotal transcriptional coactivator of nuclear receptors in vivo.",
author = "Kim, {Seung Whan} and Cheolho Cheong and Sohn, {Young Chang} and Goo, {Young Hwa} and Oh, {Wan Je} and Park, {Jung Hwan} and Joe, {So Young} and Kang, {Hyen Sam} and Kim, {Duk Kyung} and Changwon Kee and Lee, {Jae Woon} and Lee, {Han Woong}",
year = "2002",
month = "12",
day = "1",
doi = "10.1128/MCB.22.24.8409-8414.2002",
language = "English",
volume = "22",
pages = "8409--8414",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "24",

}

Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice : Implication for posterior lenticonus with cataract. / Kim, Seung Whan; Cheong, Cheolho; Sohn, Young Chang; Goo, Young Hwa; Oh, Wan Je; Park, Jung Hwan; Joe, So Young; Kang, Hyen Sam; Kim, Duk Kyung; Kee, Changwon; Lee, Jae Woon; Lee, Han Woong.

In: Molecular and Cellular Biology, Vol. 22, No. 24, 01.12.2002, p. 8409-8414.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice

T2 - Implication for posterior lenticonus with cataract

AU - Kim, Seung Whan

AU - Cheong, Cheolho

AU - Sohn, Young Chang

AU - Goo, Young Hwa

AU - Oh, Wan Je

AU - Park, Jung Hwan

AU - Joe, So Young

AU - Kang, Hyen Sam

AU - Kim, Duk Kyung

AU - Kee, Changwon

AU - Lee, Jae Woon

AU - Lee, Han Woong

PY - 2002/12/1

Y1 - 2002/12/1

N2 - ASC-2, a recently isolated transcriptional coactivator molecule, stimulates transactivation by multiple transcription factors, including nuclear receptors. We generated a potent dominant negative fragment of ASC-2, encompassing the N-terminal LXXLL motif that binds a broad range of nuclear receptors. This fragment, termed DN1, specifically inhibited endogenous ASC-2 from binding these receptors in vivo, whereas DN1/m, in which the LXXLL motif was mutated to LXXAA to abolish the receptor interactions, was inert. Interestingly, DN1 transgenic mice but not DN1/m transgenic mice exhibited severe microphthalmia and posterior lenticonus with cataract as well as a variety of pathophysiological phenotypes in many other organs. Our results provide a novel insight into the molecular and histopathological mechanism of posterior lenticonus with cataract and attest to the importance of ASC-2 as a pivotal transcriptional coactivator of nuclear receptors in vivo.

AB - ASC-2, a recently isolated transcriptional coactivator molecule, stimulates transactivation by multiple transcription factors, including nuclear receptors. We generated a potent dominant negative fragment of ASC-2, encompassing the N-terminal LXXLL motif that binds a broad range of nuclear receptors. This fragment, termed DN1, specifically inhibited endogenous ASC-2 from binding these receptors in vivo, whereas DN1/m, in which the LXXLL motif was mutated to LXXAA to abolish the receptor interactions, was inert. Interestingly, DN1 transgenic mice but not DN1/m transgenic mice exhibited severe microphthalmia and posterior lenticonus with cataract as well as a variety of pathophysiological phenotypes in many other organs. Our results provide a novel insight into the molecular and histopathological mechanism of posterior lenticonus with cataract and attest to the importance of ASC-2 as a pivotal transcriptional coactivator of nuclear receptors in vivo.

UR - http://www.scopus.com/inward/record.url?scp=18744403340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18744403340&partnerID=8YFLogxK

U2 - 10.1128/MCB.22.24.8409-8414.2002

DO - 10.1128/MCB.22.24.8409-8414.2002

M3 - Article

VL - 22

SP - 8409

EP - 8414

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 24

ER -