Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum

Mi Chung Suh, Hye Sun Cho, Youn Sung Kim, Jang Ryol Liu, Hyunsook Pai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Five cDNA clones encoding the catalytic subunits of Ser/Thr protein phosphatases (PP) of Nicotiana tabacum were described. Among them, three clones (NPP1, NPP2, and NPP3) encoded type 1 PP (PP1), whereas the rest of the clones (NPP4 and NPP5) encoded type 2A PP (PP2A). These cDNA clones exhibited high sequence identity in the PP core region to the corresponding genes from animals and plants. NPP1 mRNA was predominantly expressed in flowers, whereas NPP5 mRNA was mainly detected in leaves and flowers. Inn contrast, the transcripts of NPP2, NPP3, and NPP4 genes were present in all tissues examined. Throughout flower development, NPP1, NPP2, NPP5 mRNAs were expressed without any significant variation at the steady-state level. Genomic Southern blot showed that tobacco genome contained multiple genes and/or pseudogenes for both type 1 and type 2A phosphatases.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalPlant Molecular Biology
Volume36
Issue number2
DOIs
Publication statusPublished - 1998 Jan 1

Fingerprint

phosphoprotein phosphatase
Gene encoding
Phosphoprotein Phosphatases
Nicotiana tabacum
Tobacco
Clone Cells
Genes
clones
Messenger RNA
genes
Complementary DNA
flowers
Protein Phosphatase 1
Protein Phosphatase 2
Plant Genes
Pseudogenes
pseudogenes
protein subunits
Southern Blotting
Phosphoric Monoester Hydrolases

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Suh, Mi Chung ; Cho, Hye Sun ; Kim, Youn Sung ; Liu, Jang Ryol ; Pai, Hyunsook. / Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum. In: Plant Molecular Biology. 1998 ; Vol. 36, No. 2. pp. 315-322.
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Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum. / Suh, Mi Chung; Cho, Hye Sun; Kim, Youn Sung; Liu, Jang Ryol; Pai, Hyunsook.

In: Plant Molecular Biology, Vol. 36, No. 2, 01.01.1998, p. 315-322.

Research output: Contribution to journalArticle

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AU - Cho, Hye Sun

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