Multiple target loci assembly sequencing (mTAS)

Hyojun Han, Jung Ki Yoon, Byoung Chul Cho, Hwangbeom Kim, Duhee Bang

Research output: Contribution to journalArticlepeer-review

Abstract

Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.

Original languageEnglish
Pages (from-to)218-220
Number of pages3
JournalAnalytical Biochemistry
Volume415
Issue number2
DOIs
Publication statusPublished - 2011 Aug 15

Bibliographical note

Funding Information:
This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A101259-1001-0000100), and BK21 program of the Ministry of Education, Science, and Technology. D.B. is a TJ Park junior faculty fellow supported by the Posco TJ Park Foundation.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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