Multiplexed single-cell RNA-seq via transient barcoding for drug screening

Dongju Shin; Wookjae Lee; Ji Hyun Lee; Duhee Bang

doi:10.1101/359851

Multiplexed single-cell RNA-seq via transient barcoding for drug screening

Dongju Shin, Wookjae Lee, Ji Hyun Lee, Duhee Bang

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

To simultaneously analyze multiple samples of various conditions with scRNA-seq, we developed a universal sample barcoding method through transient transfection of SBOs. A 48-plex drug treatment experiment of pooled samples analyzed by a single run of Drop-Seq revealed a unique transcriptome response for each drug and target-specific gene expression signatures at the single-cell level. Our cost-effective method is widely applicable for single-cell profiling of multiple experimental conditions.

Original language	English
Journal	Unknown Journal
DOIs	https://doi.org/10.1101/359851
Publication status	Published - 2018 Jun 30

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
Immunology and Microbiology(all)
Neuroscience(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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abstract = "To simultaneously analyze multiple samples of various conditions with scRNA-seq, we developed a universal sample barcoding method through transient transfection of SBOs. A 48-plex drug treatment experiment of pooled samples analyzed by a single run of Drop-Seq revealed a unique transcriptome response for each drug and target-specific gene expression signatures at the single-cell level. Our cost-effective method is widely applicable for single-cell profiling of multiple experimental conditions.",

author = "Dongju Shin and Wookjae Lee and Lee, {Ji Hyun} and Duhee Bang",

note = "Publisher Copyright: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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