Multiplexed single-cell RNA-seq via transient barcoding for drug screening

Dongju Shin, Wookjae Lee, Ji Hyun Lee, Duhee Bang

Research output: Contribution to journalArticlepeer-review

Abstract

To simultaneously analyze multiple samples of various conditions with scRNA-seq, we developed a universal sample barcoding method through transient transfection of SBOs. A 48-plex drug treatment experiment of pooled samples analyzed by a single run of Drop-Seq revealed a unique transcriptome response for each drug and target-specific gene expression signatures at the single-cell level. Our cost-effective method is widely applicable for single-cell profiling of multiple experimental conditions.

Original languageEnglish
JournalUnknown Journal
DOIs
Publication statusPublished - 2018 Jun 30

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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