Multiplexing enhancement for the detection of multiple pathogen DNA

Daehoon Han, Jinkee Hong, Hyun Cheol Kim, Jong Hwan Sung, Jong Bum Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Various methods for the detection of pathogens have been researched and developed. However, most of detection methods are cost-ineffective and laborious. To minimize costs and labor, multidetection of pathogen have been widely used. In this paper, we propose a novel multi-detection method, which can enhance multi-detection capability using fluorescent dye labeled DNA nanostructures that is named DNA nanobarcodes. By using three fluorescence colors, multi-detection capability is significantly increased because of the increased combination of three colors. Moreover, our approach uses a relatively simple DNA nanostructure to precisely control the fluorescence intensity ratio. Therefore, high multi-detection ability is achieved without constructing a complicated DNA nanostructure probe. Our novel detection method can overcome the obstacles of conventional methods and enhance multi-detection capability effectively. By using our new system, we were able to successfully detect nine different DNA pathogens simultaneously. Our system can easily increase the multiplexibility by using more fluosrescent colors on DNA nanobarcode.

Original languageEnglish
Pages (from-to)7295-7299
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume13
Issue number11
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

pathogens
Pathogens
multiplexing
Multiplexing
DNA
deoxyribonucleic acid
Nanostructures
augmentation
Color
Fluorescence
Costs and Cost Analysis
color
DNA Probes
Fluorescent Dyes
Costs
costs
fluorescence
labor
Personnel
Dyes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Han, Daehoon ; Hong, Jinkee ; Kim, Hyun Cheol ; Sung, Jong Hwan ; Lee, Jong Bum. / Multiplexing enhancement for the detection of multiple pathogen DNA. In: Journal of Nanoscience and Nanotechnology. 2013 ; Vol. 13, No. 11. pp. 7295-7299.
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Multiplexing enhancement for the detection of multiple pathogen DNA. / Han, Daehoon; Hong, Jinkee; Kim, Hyun Cheol; Sung, Jong Hwan; Lee, Jong Bum.

In: Journal of Nanoscience and Nanotechnology, Vol. 13, No. 11, 01.11.2013, p. 7295-7299.

Research output: Contribution to journalArticle

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