Multivalent DNA-based vectors for DNA vaccine delivery

Young Hoon Roh, Kwang Lee, Jessica Jane Ye, Dan Luo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

DNA can be utilized as a generic delivery vector as well as a traditional biological material for DNA vaccination. Although the use of DNA as an antigen expression vector or a vaccine adjuvant has been intensively studied for several decades, the use of DNA molecules as a delivery carrier has not been explored until recently. This issue is probably due to the topological limitation of DNA in its natural linear or circular structure form. Multivalent DNA-based vector delivery platforms overcome this structural barrier and are particularly suited for DNA vaccine delivery because of their multifunctionality, monodispersity, anisotropicity, and bioconjugation ability with numerous functional moieties. In this chapter, we mainly describe the construction of multivalent DNA-based delivery vectors using DNA engineering methods. Specifically, the synthesis strategies for highly branched dendrimer-like DNA structures in general and methods for their application to DNA vaccine delivery are introduced.

Original languageEnglish
Pages (from-to)159-179
Number of pages21
JournalMethods in Molecular Biology
Volume1143
DOIs
Publication statusPublished - 2014 Jan 1

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DNA Vaccines
DNA
Dendrimers
Vaccination
Vaccines
Antigens

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Roh, Young Hoon ; Lee, Kwang ; Ye, Jessica Jane ; Luo, Dan. / Multivalent DNA-based vectors for DNA vaccine delivery. In: Methods in Molecular Biology. 2014 ; Vol. 1143. pp. 159-179.
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Multivalent DNA-based vectors for DNA vaccine delivery. / Roh, Young Hoon; Lee, Kwang; Ye, Jessica Jane; Luo, Dan.

In: Methods in Molecular Biology, Vol. 1143, 01.01.2014, p. 159-179.

Research output: Contribution to journalArticle

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