TY - JOUR
T1 - Multivalent DNA-based vectors for DNA vaccine delivery
AU - Roh, Young Hoon
AU - Lee, Kwang
AU - Ye, Jessica Jane
AU - Luo, Dan
PY - 2014/1/1
Y1 - 2014/1/1
N2 - DNA can be utilized as a generic delivery vector as well as a traditional biological material for DNA vaccination. Although the use of DNA as an antigen expression vector or a vaccine adjuvant has been intensively studied for several decades, the use of DNA molecules as a delivery carrier has not been explored until recently. This issue is probably due to the topological limitation of DNA in its natural linear or circular structure form. Multivalent DNA-based vector delivery platforms overcome this structural barrier and are particularly suited for DNA vaccine delivery because of their multifunctionality, monodispersity, anisotropicity, and bioconjugation ability with numerous functional moieties. In this chapter, we mainly describe the construction of multivalent DNA-based delivery vectors using DNA engineering methods. Specifically, the synthesis strategies for highly branched dendrimer-like DNA structures in general and methods for their application to DNA vaccine delivery are introduced.
AB - DNA can be utilized as a generic delivery vector as well as a traditional biological material for DNA vaccination. Although the use of DNA as an antigen expression vector or a vaccine adjuvant has been intensively studied for several decades, the use of DNA molecules as a delivery carrier has not been explored until recently. This issue is probably due to the topological limitation of DNA in its natural linear or circular structure form. Multivalent DNA-based vector delivery platforms overcome this structural barrier and are particularly suited for DNA vaccine delivery because of their multifunctionality, monodispersity, anisotropicity, and bioconjugation ability with numerous functional moieties. In this chapter, we mainly describe the construction of multivalent DNA-based delivery vectors using DNA engineering methods. Specifically, the synthesis strategies for highly branched dendrimer-like DNA structures in general and methods for their application to DNA vaccine delivery are introduced.
UR - http://www.scopus.com/inward/record.url?scp=84910139259&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84910139259&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0410-5_11
DO - 10.1007/978-1-4939-0410-5_11
M3 - Article
C2 - 24715288
AN - SCOPUS:84910139259
VL - 1143
SP - 159
EP - 179
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -