Mussel Adhesion-Inspired Reverse Transfection Platform Enhances Osteogenic Differentiation and Bone Formation of Human Adipose-Derived Stem Cells

Jisoo Shin, Jung Ho Cho, Yoonhee Jin, Kisuk Yang, Jong Seung Lee, Hyun Ji Park, Hyung Seop Han, Jinkyu Lee, Hojeong Jeon, Heungsoo Shin, Seung-Woo Cho

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Using small interfering RNA (siRNA) to regulate gene expression is an emerging strategy for stem cell manipulation to improve stem cell therapy. However, conventional methods of siRNA delivery into stem cells based on solution-mediated transfection are limited due to low transfection efficiency and insufficient duration of cell-siRNA contact during lengthy culturing protocols. To overcome these limitations, a bio-inspired polymer-mediated reverse transfection system is developed consisting of implantable poly(lactic-co-glycolic acid) (PLGA) scaffolds functionalized with siRNA-lipidoid nanoparticle (sLNP) complexes via polydopamine (pDA) coating. Immobilized sLNP complexes are stably maintained without any loss of siRNA on the pDA-coated scaffolds for 2 weeks, likely due to the formation of strong covalent bonds between amine groups of sLNP and catechol group of pDA. siRNA reverse transfection with the pDA-sLNP-PLGA system does not exhibit cytotoxicity and induces efficient silencing of an osteogenesis inhibitor gene in human adipose-derived stem cells (hADSCs), resulting in enhanced osteogenic differentiation of hADSCs. Finally, hADSCs osteogenically committed on the pDA-sLNP-PLGA scaffolds enhanced bone formation in a mouse model of critical-sized bone defect. Therefore, the bio-inspired reverse transfection system can provide an all-in-one platform for genetic modification, differentiation, and transplantation of stem cells, simultaneously enabling both stem cell manipulation and tissue engineering.

Original languageEnglish
Pages (from-to)6266-6278
Number of pages13
JournalSmall
Volume12
Issue number45
DOIs
Publication statusPublished - 2016 Dec 7

Fingerprint

Bivalvia
Stem cells
Osteogenesis
Small Interfering RNA
Transfection
Bone
Stem Cells
Adhesion
RNA
Nanoparticles
Scaffolds
Acids
Covalent bonds
Cell Engineering
Cytotoxicity
Tissue engineering
Gene expression
Stem Cell Transplantation
Amines
Tissue Engineering

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Shin, Jisoo ; Cho, Jung Ho ; Jin, Yoonhee ; Yang, Kisuk ; Lee, Jong Seung ; Park, Hyun Ji ; Han, Hyung Seop ; Lee, Jinkyu ; Jeon, Hojeong ; Shin, Heungsoo ; Cho, Seung-Woo. / Mussel Adhesion-Inspired Reverse Transfection Platform Enhances Osteogenic Differentiation and Bone Formation of Human Adipose-Derived Stem Cells. In: Small. 2016 ; Vol. 12, No. 45. pp. 6266-6278.
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Mussel Adhesion-Inspired Reverse Transfection Platform Enhances Osteogenic Differentiation and Bone Formation of Human Adipose-Derived Stem Cells. / Shin, Jisoo; Cho, Jung Ho; Jin, Yoonhee; Yang, Kisuk; Lee, Jong Seung; Park, Hyun Ji; Han, Hyung Seop; Lee, Jinkyu; Jeon, Hojeong; Shin, Heungsoo; Cho, Seung-Woo.

In: Small, Vol. 12, No. 45, 07.12.2016, p. 6266-6278.

Research output: Contribution to journalArticle

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AU - Shin, Jisoo

AU - Cho, Jung Ho

AU - Jin, Yoonhee

AU - Yang, Kisuk

AU - Lee, Jong Seung

AU - Park, Hyun Ji

AU - Han, Hyung Seop

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AU - Jeon, Hojeong

AU - Shin, Heungsoo

AU - Cho, Seung-Woo

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