Mussel-inspired cell-adhesion peptide modification for enhanced endothelialization of decellularized blood vessels

Enhanced endothelialization of tissue-engineered blood vessels is essential for vascular regeneration and function of engineered vessels. In this study, mussel-inspired surface chemistry of polydopamine (pDA) coatings are applied to functionalize decellularized vein matrix (DVM) with extracellular matrix-derived cell adhesion peptides (RGD and YIGSR). DVMs engineered with pDA-peptides enhance focal adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells (EPCs) derived from cord blood and embryonic stem cells compared with EPCs on non-coated or pDA-coated DVMs. These results indicate that pDA-peptide functionalization may contribute to enhanced, rapid endothelialization of DVM surfaces by promoting adhesion, proliferation, and differentiation of circulating EPCs. Ultimately, this approach may be useful for improving in vivo patency and function of decellularized matrix-based blood vessels. Mussel-inspired catechol functionalization by polydopamine coating leads to efficient immobilization of cell adhesion peptides on decellularized blood vessel matrices. Peptide modification promotes adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells, which ultimately facilitates endothelialization of the decellularized matrices.