TY - JOUR
T1 - Mussel-inspired cell-adhesion peptide modification for enhanced endothelialization of decellularized blood vessels
AU - Lee, Jung Seung
AU - Lee, Kihong
AU - Moon, Sung Hwan
AU - Chung, Hyung Min
AU - Lee, Jun Hyup
AU - Um, Soong Ho
AU - Kim, Dong Ik
AU - Cho, Seung Woo
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/8
Y1 - 2014/8
N2 - Enhanced endothelialization of tissue-engineered blood vessels is essential for vascular regeneration and function of engineered vessels. In this study, mussel-inspired surface chemistry of polydopamine (pDA) coatings are applied to functionalize decellularized vein matrix (DVM) with extracellular matrix-derived cell adhesion peptides (RGD and YIGSR). DVMs engineered with pDA-peptides enhance focal adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells (EPCs) derived from cord blood and embryonic stem cells compared with EPCs on non-coated or pDA-coated DVMs. These results indicate that pDA-peptide functionalization may contribute to enhanced, rapid endothelialization of DVM surfaces by promoting adhesion, proliferation, and differentiation of circulating EPCs. Ultimately, this approach may be useful for improving in vivo patency and function of decellularized matrix-based blood vessels. Mussel-inspired catechol functionalization by polydopamine coating leads to efficient immobilization of cell adhesion peptides on decellularized blood vessel matrices. Peptide modification promotes adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells, which ultimately facilitates endothelialization of the decellularized matrices.
AB - Enhanced endothelialization of tissue-engineered blood vessels is essential for vascular regeneration and function of engineered vessels. In this study, mussel-inspired surface chemistry of polydopamine (pDA) coatings are applied to functionalize decellularized vein matrix (DVM) with extracellular matrix-derived cell adhesion peptides (RGD and YIGSR). DVMs engineered with pDA-peptides enhance focal adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells (EPCs) derived from cord blood and embryonic stem cells compared with EPCs on non-coated or pDA-coated DVMs. These results indicate that pDA-peptide functionalization may contribute to enhanced, rapid endothelialization of DVM surfaces by promoting adhesion, proliferation, and differentiation of circulating EPCs. Ultimately, this approach may be useful for improving in vivo patency and function of decellularized matrix-based blood vessels. Mussel-inspired catechol functionalization by polydopamine coating leads to efficient immobilization of cell adhesion peptides on decellularized blood vessel matrices. Peptide modification promotes adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells, which ultimately facilitates endothelialization of the decellularized matrices.
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U2 - 10.1002/mabi.201400052
DO - 10.1002/mabi.201400052
M3 - Article
C2 - 24831738
AN - SCOPUS:84906315910
VL - 14
SP - 1181
EP - 1189
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
SN - 1616-5187
IS - 8
ER -