Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis

Maxence S. Macia, Jan Halbritter, Marion Delous, Cecilie Bredrup, Arthur Gutter, Emilie Filhol, Anne E.C. Mellgren, Sabine Leh, Albane Bizet, Daniela A. Braun, Heon Yung Gee, Flora Silbermann, Charline Henry, Pauline Krug, Christine Bole-Feysot, Patrick Nitschké, Dominique Joly, Philippe Nicoud, André Paget, Heidi HauglandDamien Brackmann, Nayir Ahmet, Richard Sandford, Nurcan Cengiz, Per M. Knappskog, Helge Boman, Bolan Linghu, Fan Yang, Edward J. Oakeley, Pierre Saint Mézard, Andreas W. Sailer, Stefan Johansson, Eyvind Rødahl, Sophie Saunier, Friedhelm Hildebrandt, Alexandre Benmerah

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nephronophthisis (NPH), an autosomal-recessive tubulointerstitial nephritis, is the most common cause of hereditary end-stage renal disease in the first three decades of life. Since most NPH gene products (NPHP) function at the primary cilium, NPH is classified as a ciliopathy. We identified mutations in a candidate gene in eight individuals from five families presenting late-onset NPH with massive renal fibrosis. This gene encodes MAPKBP1, a poorly characterized scaffolding protein for JNK signaling. Immunofluorescence analyses showed that MAPKBP1 is not present at the primary cilium and that fibroblasts from affected individuals did not display ciliogenesis defects, indicating that MAPKBP1 may represent a new family of NPHP not involved in cilia-associated functions. Instead, MAPKBP1 is recruited to mitotic spindle poles (MSPs) during the early phases of mitosis where it colocalizes with its paralog WDR62, which plays a key role at MSP. Detected mutations compromise recruitment of MAPKBP1 to the MSP and/or its interaction with JNK2 or WDR62. Additionally, we show increased DNA damage response signaling in fibroblasts from affected individuals and upon knockdown of Mapkbp1 in murine cell lines, a phenotype previously associated with NPH. In conclusion, we identified mutations in MAPKBP1 as a genetic cause of juvenile or late-onset and cilia-independent NPH.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalAmerican Journal of Human Genetics
Volume100
Issue number2
DOIs
Publication statusPublished - 2017 Feb 2

Fingerprint

Cilia
Spindle Poles
Spindle Apparatus
Mutation
Genes
Fibroblasts
Interstitial Nephritis
Mitosis
DNA Damage
Chronic Kidney Failure
Fluorescent Antibody Technique
Fibrosis
Phenotype
Kidney
Cell Line
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Macia, M. S., Halbritter, J., Delous, M., Bredrup, C., Gutter, A., Filhol, E., ... Benmerah, A. (2017). Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis. American Journal of Human Genetics, 100(2), 323-333. https://doi.org/10.1016/j.ajhg.2016.12.011
Macia, Maxence S. ; Halbritter, Jan ; Delous, Marion ; Bredrup, Cecilie ; Gutter, Arthur ; Filhol, Emilie ; Mellgren, Anne E.C. ; Leh, Sabine ; Bizet, Albane ; Braun, Daniela A. ; Gee, Heon Yung ; Silbermann, Flora ; Henry, Charline ; Krug, Pauline ; Bole-Feysot, Christine ; Nitschké, Patrick ; Joly, Dominique ; Nicoud, Philippe ; Paget, André ; Haugland, Heidi ; Brackmann, Damien ; Ahmet, Nayir ; Sandford, Richard ; Cengiz, Nurcan ; Knappskog, Per M. ; Boman, Helge ; Linghu, Bolan ; Yang, Fan ; Oakeley, Edward J. ; Saint Mézard, Pierre ; Sailer, Andreas W. ; Johansson, Stefan ; Rødahl, Eyvind ; Saunier, Sophie ; Hildebrandt, Friedhelm ; Benmerah, Alexandre. / Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis. In: American Journal of Human Genetics. 2017 ; Vol. 100, No. 2. pp. 323-333.
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abstract = "Nephronophthisis (NPH), an autosomal-recessive tubulointerstitial nephritis, is the most common cause of hereditary end-stage renal disease in the first three decades of life. Since most NPH gene products (NPHP) function at the primary cilium, NPH is classified as a ciliopathy. We identified mutations in a candidate gene in eight individuals from five families presenting late-onset NPH with massive renal fibrosis. This gene encodes MAPKBP1, a poorly characterized scaffolding protein for JNK signaling. Immunofluorescence analyses showed that MAPKBP1 is not present at the primary cilium and that fibroblasts from affected individuals did not display ciliogenesis defects, indicating that MAPKBP1 may represent a new family of NPHP not involved in cilia-associated functions. Instead, MAPKBP1 is recruited to mitotic spindle poles (MSPs) during the early phases of mitosis where it colocalizes with its paralog WDR62, which plays a key role at MSP. Detected mutations compromise recruitment of MAPKBP1 to the MSP and/or its interaction with JNK2 or WDR62. Additionally, we show increased DNA damage response signaling in fibroblasts from affected individuals and upon knockdown of Mapkbp1 in murine cell lines, a phenotype previously associated with NPH. In conclusion, we identified mutations in MAPKBP1 as a genetic cause of juvenile or late-onset and cilia-independent NPH.",
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Macia, MS, Halbritter, J, Delous, M, Bredrup, C, Gutter, A, Filhol, E, Mellgren, AEC, Leh, S, Bizet, A, Braun, DA, Gee, HY, Silbermann, F, Henry, C, Krug, P, Bole-Feysot, C, Nitschké, P, Joly, D, Nicoud, P, Paget, A, Haugland, H, Brackmann, D, Ahmet, N, Sandford, R, Cengiz, N, Knappskog, PM, Boman, H, Linghu, B, Yang, F, Oakeley, EJ, Saint Mézard, P, Sailer, AW, Johansson, S, Rødahl, E, Saunier, S, Hildebrandt, F & Benmerah, A 2017, 'Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis', American Journal of Human Genetics, vol. 100, no. 2, pp. 323-333. https://doi.org/10.1016/j.ajhg.2016.12.011

Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis. / Macia, Maxence S.; Halbritter, Jan; Delous, Marion; Bredrup, Cecilie; Gutter, Arthur; Filhol, Emilie; Mellgren, Anne E.C.; Leh, Sabine; Bizet, Albane; Braun, Daniela A.; Gee, Heon Yung; Silbermann, Flora; Henry, Charline; Krug, Pauline; Bole-Feysot, Christine; Nitschké, Patrick; Joly, Dominique; Nicoud, Philippe; Paget, André; Haugland, Heidi; Brackmann, Damien; Ahmet, Nayir; Sandford, Richard; Cengiz, Nurcan; Knappskog, Per M.; Boman, Helge; Linghu, Bolan; Yang, Fan; Oakeley, Edward J.; Saint Mézard, Pierre; Sailer, Andreas W.; Johansson, Stefan; Rødahl, Eyvind; Saunier, Sophie; Hildebrandt, Friedhelm; Benmerah, Alexandre.

In: American Journal of Human Genetics, Vol. 100, No. 2, 02.02.2017, p. 323-333.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis

AU - Macia, Maxence S.

AU - Halbritter, Jan

AU - Delous, Marion

AU - Bredrup, Cecilie

AU - Gutter, Arthur

AU - Filhol, Emilie

AU - Mellgren, Anne E.C.

AU - Leh, Sabine

AU - Bizet, Albane

AU - Braun, Daniela A.

AU - Gee, Heon Yung

AU - Silbermann, Flora

AU - Henry, Charline

AU - Krug, Pauline

AU - Bole-Feysot, Christine

AU - Nitschké, Patrick

AU - Joly, Dominique

AU - Nicoud, Philippe

AU - Paget, André

AU - Haugland, Heidi

AU - Brackmann, Damien

AU - Ahmet, Nayir

AU - Sandford, Richard

AU - Cengiz, Nurcan

AU - Knappskog, Per M.

AU - Boman, Helge

AU - Linghu, Bolan

AU - Yang, Fan

AU - Oakeley, Edward J.

AU - Saint Mézard, Pierre

AU - Sailer, Andreas W.

AU - Johansson, Stefan

AU - Rødahl, Eyvind

AU - Saunier, Sophie

AU - Hildebrandt, Friedhelm

AU - Benmerah, Alexandre

PY - 2017/2/2

Y1 - 2017/2/2

N2 - Nephronophthisis (NPH), an autosomal-recessive tubulointerstitial nephritis, is the most common cause of hereditary end-stage renal disease in the first three decades of life. Since most NPH gene products (NPHP) function at the primary cilium, NPH is classified as a ciliopathy. We identified mutations in a candidate gene in eight individuals from five families presenting late-onset NPH with massive renal fibrosis. This gene encodes MAPKBP1, a poorly characterized scaffolding protein for JNK signaling. Immunofluorescence analyses showed that MAPKBP1 is not present at the primary cilium and that fibroblasts from affected individuals did not display ciliogenesis defects, indicating that MAPKBP1 may represent a new family of NPHP not involved in cilia-associated functions. Instead, MAPKBP1 is recruited to mitotic spindle poles (MSPs) during the early phases of mitosis where it colocalizes with its paralog WDR62, which plays a key role at MSP. Detected mutations compromise recruitment of MAPKBP1 to the MSP and/or its interaction with JNK2 or WDR62. Additionally, we show increased DNA damage response signaling in fibroblasts from affected individuals and upon knockdown of Mapkbp1 in murine cell lines, a phenotype previously associated with NPH. In conclusion, we identified mutations in MAPKBP1 as a genetic cause of juvenile or late-onset and cilia-independent NPH.

AB - Nephronophthisis (NPH), an autosomal-recessive tubulointerstitial nephritis, is the most common cause of hereditary end-stage renal disease in the first three decades of life. Since most NPH gene products (NPHP) function at the primary cilium, NPH is classified as a ciliopathy. We identified mutations in a candidate gene in eight individuals from five families presenting late-onset NPH with massive renal fibrosis. This gene encodes MAPKBP1, a poorly characterized scaffolding protein for JNK signaling. Immunofluorescence analyses showed that MAPKBP1 is not present at the primary cilium and that fibroblasts from affected individuals did not display ciliogenesis defects, indicating that MAPKBP1 may represent a new family of NPHP not involved in cilia-associated functions. Instead, MAPKBP1 is recruited to mitotic spindle poles (MSPs) during the early phases of mitosis where it colocalizes with its paralog WDR62, which plays a key role at MSP. Detected mutations compromise recruitment of MAPKBP1 to the MSP and/or its interaction with JNK2 or WDR62. Additionally, we show increased DNA damage response signaling in fibroblasts from affected individuals and upon knockdown of Mapkbp1 in murine cell lines, a phenotype previously associated with NPH. In conclusion, we identified mutations in MAPKBP1 as a genetic cause of juvenile or late-onset and cilia-independent NPH.

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