Mutiscale substrates based on hydrogel-incorporated silicon nanowires for protein patterning and microarray-based immunoassays

Sang Won Han, Seulah Lee, Juree Hong, Eunji Jang, Taeyoon Lee, Won-Gun Koh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Here, protein micropatterns were prepared on micropatterned nanostructures for potential applications in microarray-based multiplex bioassays with enhanced protein-loading capacity and detection sensitivity. Vertically-aligned silicon nanowires (SiNWs) that were about 8. μm in height and 150. nm in diameter were prepared using an etching process and were surface-modified with aminopropyltriethoxysilane (APTES) to allow them to covalently immobilize proteins. The SiNW substrate was then overlaid with a micropattern of poly(ethylene glycol) (PEG) hydrogel to create defined arrays of microwells consisting of APTES-modified SiNW on the bottom of the wells, with hydrogel on the walls of the wells. Due to the non-adhesiveness of PEG hydrogels toward proteins, proteins were selectively immobilized on the surface-modified SiNW regions to create protein micropatterns. The increase in surface area increased the protein loading capacity of the SiNWs by more than 10 times the capacity of a planar silicon substrate. Immunobinding assays between IgG and anti-IgG and between IgM and anti-IgM that were performed on micropatterned SiNWs emitted stronger fluorescent signals and showed higher sensitivity than assays performed on planar silicon substrates. Finally, microfluidic channels were successfully integrated into the micropatterned SiNWs to enable the simultaneous performance of multiple immunoassays on a single microarray platform.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalBiosensors and Bioelectronics
Volume45
Issue number1
DOIs
Publication statusPublished - 2013 Jul 5

Fingerprint

Nanowires
Protein Array Analysis
Hydrogel
Silicon
Microarrays
Immunoassay
Hydrogels
Proteins
Substrates
Polyethylene glycols
Assays
Microfluidics
Ethylene Glycol
Nanostructures
Bioassay
Biological Assay
Immunoglobulin M
Etching
Immunoglobulin G

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "Here, protein micropatterns were prepared on micropatterned nanostructures for potential applications in microarray-based multiplex bioassays with enhanced protein-loading capacity and detection sensitivity. Vertically-aligned silicon nanowires (SiNWs) that were about 8. μm in height and 150. nm in diameter were prepared using an etching process and were surface-modified with aminopropyltriethoxysilane (APTES) to allow them to covalently immobilize proteins. The SiNW substrate was then overlaid with a micropattern of poly(ethylene glycol) (PEG) hydrogel to create defined arrays of microwells consisting of APTES-modified SiNW on the bottom of the wells, with hydrogel on the walls of the wells. Due to the non-adhesiveness of PEG hydrogels toward proteins, proteins were selectively immobilized on the surface-modified SiNW regions to create protein micropatterns. The increase in surface area increased the protein loading capacity of the SiNWs by more than 10 times the capacity of a planar silicon substrate. Immunobinding assays between IgG and anti-IgG and between IgM and anti-IgM that were performed on micropatterned SiNWs emitted stronger fluorescent signals and showed higher sensitivity than assays performed on planar silicon substrates. Finally, microfluidic channels were successfully integrated into the micropatterned SiNWs to enable the simultaneous performance of multiple immunoassays on a single microarray platform.",
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Mutiscale substrates based on hydrogel-incorporated silicon nanowires for protein patterning and microarray-based immunoassays. / Han, Sang Won; Lee, Seulah; Hong, Juree; Jang, Eunji; Lee, Taeyoon; Koh, Won-Gun.

In: Biosensors and Bioelectronics, Vol. 45, No. 1, 05.07.2013, p. 129-135.

Research output: Contribution to journalArticle

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