Mycobacterium abscessus activates the macrophage innate immune response via a physical and functional interaction between TLR2 and dectin-1

Dong Min Shin, Chul Su Yang, Jae Min Yuk, Ji Yeon Lee, Ki Hye Kim, SungJae Shin, Kazuhiko Takahara, Sung Joong Lee, Eun Kyeong Jo

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Abstract

Mycobacterium abscessus (Mab) is an emerging and rapidly growing non-tuberculous mycobacterium (NTM). Compared with M. tuberculosis, which is responsible for tuberculosis, much less is known about NTM-induced innate immune mechanisms. Here we investigated the involvement of pattern-recognition receptors and associated signalling in Mab-mediated innate immune responses. Mab activated the extracellular signal-regulated kinase (ERK)1/2 and p38 mitogen-activated protein kinases (MAPKs), and induced the secretion of tumour necrosis factor-α, interleukin (IL)-6 and IL-12p40 in murine macrophages via Toll-like receptor (TLR) 2. Notably, the activation of ERK1/2, but not p38, was crucial for Mab-induced pro-inflammatory cytokine production. The ITAM-like motif of dectin-1 critically contributed to Mab internalization and cytokine secretion by macrophages. In addition, dectin-1, in cooperation with TLR2, was required for the efficient phagocytosis of Mab, ERK1/2 activation and pro-inflammatory cytokine secretion. Co-immunoprecipitation and confocal analysis showed the physical interaction and colocalization of dectin-1 with TLR2 following Mab stimulation. Moreover, dectin-1-induced Syk activation was essential for the production of inflammatory cytokines and the release of reactive oxygen species by Mab-infected macrophages. Collectively, these data demonstrate that Mab actively internalizes into and robustly activates innate immune responses in macrophages through a physical and functional interaction between TLR2 and dectin-1.

Original languageEnglish
Pages (from-to)1608-1621
Number of pages14
JournalCellular Microbiology
Volume10
Issue number8
DOIs
Publication statusPublished - 2008 Aug 1

Fingerprint

Mycobacterium
Innate Immunity
Macrophages
Cytokines
Tuberculosis
dectin 1
Pattern Recognition Receptors
Toll-Like Receptor 2
Mitogen-Activated Protein Kinase 3
Interleukins
Mitogen-Activated Protein Kinase 1
p38 Mitogen-Activated Protein Kinases
Phagocytosis
Immunoprecipitation
Interleukin-6
Reactive Oxygen Species
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Shin, Dong Min ; Yang, Chul Su ; Yuk, Jae Min ; Lee, Ji Yeon ; Kim, Ki Hye ; Shin, SungJae ; Takahara, Kazuhiko ; Lee, Sung Joong ; Jo, Eun Kyeong. / Mycobacterium abscessus activates the macrophage innate immune response via a physical and functional interaction between TLR2 and dectin-1. In: Cellular Microbiology. 2008 ; Vol. 10, No. 8. pp. 1608-1621.
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Mycobacterium abscessus activates the macrophage innate immune response via a physical and functional interaction between TLR2 and dectin-1. / Shin, Dong Min; Yang, Chul Su; Yuk, Jae Min; Lee, Ji Yeon; Kim, Ki Hye; Shin, SungJae; Takahara, Kazuhiko; Lee, Sung Joong; Jo, Eun Kyeong.

In: Cellular Microbiology, Vol. 10, No. 8, 01.08.2008, p. 1608-1621.

Research output: Contribution to journalArticle

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AU - Shin, Dong Min

AU - Yang, Chul Su

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AU - Lee, Ji Yeon

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