Mycobacterium tuberculosis ESAT6 drives the activation and maturation of bone marrow-derived dendritic cells via TLR4-mediated signaling

Ah Ra Jang, Green Kim, Jung Joo Hong, Soon Myung Kang, Sung Jae Shin, Jong Hwan Park

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

6-kDa early secretory antigenic target (ESAT6), a virulent factor of Mycobacterium tuberculosis, is involved in immune regulation. However, the underlying mechanism behind the activation and maturation of dendritic cells (DCs) by ESAT6 remains unclear. In this study, we investigated the effect on TLRs signaling on the regulation of ESAT6-induced activation and maturation of DCs. ESAT6 induced production of IL-6, TNF-α, and IL-12p40 in bone marrow- derived dendritic cells (BMDCs) from wild-type and TLR2-deficient mice, with this induction abolished in TLR4-deficient cells. NF-κB is essential for the ESAT6-induced production of the cytokines in BMDCs. TLR4 was also required for ESAT6-induced activation of NF-κB and MAPKs in BMDCs. ESAT6 additionally upregulated the expression of surface molecules CD80, CD86, and MHC-II, and also promoted the ability of CD4+ T cells to secrete IFN-γ via the TLR4-dependent pathway. Our findings suggest that TLR4 is critical in the activation and maturation of DCs in response to ESAT6.

Original languageEnglish
Article numbere13
JournalImmune Network
Volume19
Issue number2
DOIs
Publication statusPublished - 2019 Apr

Bibliographical note

Funding Information:
This study was supported by a grant (HI15C0493) from the Korean Health Technology R&D Project funded by the Ministry of Health & Welfare and a Research Initiative Program (KGM4571811) from Korea Research Institute of Bioscience and Biotechnology, Republic of Korea.

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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