Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization

Deok Rim Heo, SungJae Shin, Woo Sik Kim, Kyung Tae Noh, Jin Wook Park, Kwang Hee Son, Won Sun Park, Min Goo Lee, Daejin Kim, Yong Kyoo Shin, In Duk Jung, Yeong Min Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated naïve T cells, polarized CD4+ and CD8+ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.

Original languageEnglish
Pages (from-to)642-647
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume411
Issue number3
DOIs
Publication statusPublished - 2011 Aug 5

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Dihydrolipoamide Dehydrogenase
Mycobacterium tuberculosis
Dendritic Cells
T-cells
Polarization
Tuberculosis
Chemical activation
T-Lymphocytes
Mixed Lymphocyte Culture Test
Gene encoding
Lymphocytes
Adaptive Immunity
Infection
Pulmonary Tuberculosis
Innate Immunity
Morbidity
Mortality
Genes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Heo, Deok Rim ; Shin, SungJae ; Kim, Woo Sik ; Noh, Kyung Tae ; Park, Jin Wook ; Son, Kwang Hee ; Park, Won Sun ; Lee, Min Goo ; Kim, Daejin ; Shin, Yong Kyoo ; Jung, In Duk ; Park, Yeong Min. / Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 411, No. 3. pp. 642-647.
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abstract = "Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated na{\"i}ve T cells, polarized CD4+ and CD8+ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.",
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Heo, DR, Shin, S, Kim, WS, Noh, KT, Park, JW, Son, KH, Park, WS, Lee, MG, Kim, D, Shin, YK, Jung, ID & Park, YM 2011, 'Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization', Biochemical and Biophysical Research Communications, vol. 411, no. 3, pp. 642-647. https://doi.org/10.1016/j.bbrc.2011.07.013

Mycobacterium tuberculosis lpdC, Rv0462, induces dendritic cell maturation and Th1 polarization. / Heo, Deok Rim; Shin, SungJae; Kim, Woo Sik; Noh, Kyung Tae; Park, Jin Wook; Son, Kwang Hee; Park, Won Sun; Lee, Min Goo; Kim, Daejin; Shin, Yong Kyoo; Jung, In Duk; Park, Yeong Min.

In: Biochemical and Biophysical Research Communications, Vol. 411, No. 3, 05.08.2011, p. 642-647.

Research output: Contribution to journalArticle

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AB - Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated naïve T cells, polarized CD4+ and CD8+ T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.

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