Mycobacterium tuberculosis PE27 activates dendritic cells and contributes to Th1-polarized memory immune responses during in vivo infection

Woo Sik Kim, Jong Seok Kim, Seung Bin Cha, So Jeong Kim, Hongmin Kim, Kee Woong Kwon, Seung Jung Han, Soo Young Choi, SungJae Shin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A gradual understanding of the proline-glutamate (PE) and proline-proline-glutamate (PPE) families, which compromise 10% of the coding regions in the Mycobacterium tuberculosis (Mtb) genome, has uncovered unique roles in host–pathogen interactions. However, the immunological function of PE27 (Rv2769c), the largest PE member, remains unclear. Here, we explored the functional roles and related signaling mechanisms of PE27 in the interaction with dendritic cells (DCs) to shape the T cell response. PE27 phenotypically and functionally induces DC maturation by up-regulating CD80, CD86, MHC class I and MHC class II expression on the DC surface to promote the production of TNF-α, IL-1β, IL-6, and IL-12p70 but not IL-10. Additionally, we found that PE27-mediated DC activation requires the participation of mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NF-κB) signaling pathways. Interestingly, PE27-treated DCs directed naïve CD4+ T cells to secrete IFN-γ and activate T-bet but not GATA-3. PE27 also induced IFN-γ-producing memory T cell responses in Mtb-infected mice, indicating that PE27 contributes to Th1-polarization. Taken together, these findings suggest that PE27 possesses Th1-polarizing potential through DC maturation and could be useful in the design of TB vaccines.

Original languageEnglish
Pages (from-to)440-453
Number of pages14
JournalImmunobiology
Volume221
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

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Mycobacterium tuberculosis
Dendritic Cells
Proline
Infection
Glutamic Acid
T-Lymphocytes
Cell Shape
Mitogen-Activated Protein Kinases
Interleukin-1
Interleukin-10
Interleukin-6
Vaccines
Genome

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Kim, Woo Sik ; Kim, Jong Seok ; Cha, Seung Bin ; Kim, So Jeong ; Kim, Hongmin ; Kwon, Kee Woong ; Han, Seung Jung ; Choi, Soo Young ; Shin, SungJae. / Mycobacterium tuberculosis PE27 activates dendritic cells and contributes to Th1-polarized memory immune responses during in vivo infection. In: Immunobiology. 2016 ; Vol. 221, No. 3. pp. 440-453.
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Mycobacterium tuberculosis PE27 activates dendritic cells and contributes to Th1-polarized memory immune responses during in vivo infection. / Kim, Woo Sik; Kim, Jong Seok; Cha, Seung Bin; Kim, So Jeong; Kim, Hongmin; Kwon, Kee Woong; Han, Seung Jung; Choi, Soo Young; Shin, SungJae.

In: Immunobiology, Vol. 221, No. 3, 01.03.2016, p. 440-453.

Research output: Contribution to journalArticle

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