Mycobacterium tuberculosis Rv0652 stimulates production of tumour necrosis factor and monocytes chemoattractant protein-1 in macrophages through the Toll-like receptor 4 pathway

Kwangwook Kim, Hosung Sohn, Jong Seok Kim, Han Gyu Choi, Eui Hong Byun, Kang In Lee, Sung Jae Shin, Chang Hwa Song, Jeong Kyu Park, Hwa Jung Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mycobacterial proteins interact with host macrophages and modulate their functions and cytokine gene expression profile. The protein Rv0652 is abundant in culture filtrates of Mycobacterium tuberculosis K-strain, which belongs to the Beijing family, compared with levels in the H37Rv and CDC1551 strains. Rv0652 induces strong antibody responses in patients with active tuberculosis. We investigated pro-inflammatory cytokine production induced by Rv0652 in murine macrophages and the roles of signalling pathways. In RAW264.7 cells and bone marrow-derived macrophages, recombinant Rv0652 induced predominantly tumour necrosis factor (TNF) and monocyte chemoattractant protein (MCP)-1 production, which was dependent on mitogen-activated protein kinases and nuclear factor-κB. Specific signalling pathway inhibitors revealed that the extracellular signal-regulated kinase 1/2 (ERK1/2), p38 and phosphatidylinositol 3-kinase (PI3K) pathways were essential for Rv0652-induced TNF production, whereas the ERK1/2 and PI3K pathways, but not the p38 pathway, were critical for MCP-1 production in macrophages. Rv0652-stimulated TNF and MCP-1 secretion by macrophages occurred in a Toll-like receptor 4-dependent and MyD88-dependent manner. In addition, Rv0652 significantly up-regulated the expression of the mannose receptor, CD80, CD86 and MHC class II molecules. These results suggest that Rv0652 can induce a protective immunity against M. tuberculosis through the macrophage activation.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalImmunology
Volume136
Issue number2
DOIs
Publication statusPublished - 2012 Jun 1

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Toll-Like Receptor 4
Chemokine CCL2
Mycobacterium tuberculosis
Tumor Necrosis Factor-alpha
Macrophages
Phosphatidylinositol 3-Kinase
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Cytokines
Critical Pathways
Macrophage Activation
Mitogen-Activated Protein Kinases
Transcriptome
Antibody Formation
Immunity
Tuberculosis
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Kim, Kwangwook ; Sohn, Hosung ; Kim, Jong Seok ; Choi, Han Gyu ; Byun, Eui Hong ; Lee, Kang In ; Shin, Sung Jae ; Song, Chang Hwa ; Park, Jeong Kyu ; Kim, Hwa Jung. / Mycobacterium tuberculosis Rv0652 stimulates production of tumour necrosis factor and monocytes chemoattractant protein-1 in macrophages through the Toll-like receptor 4 pathway. In: Immunology. 2012 ; Vol. 136, No. 2. pp. 231-240.
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Mycobacterium tuberculosis Rv0652 stimulates production of tumour necrosis factor and monocytes chemoattractant protein-1 in macrophages through the Toll-like receptor 4 pathway. / Kim, Kwangwook; Sohn, Hosung; Kim, Jong Seok; Choi, Han Gyu; Byun, Eui Hong; Lee, Kang In; Shin, Sung Jae; Song, Chang Hwa; Park, Jeong Kyu; Kim, Hwa Jung.

In: Immunology, Vol. 136, No. 2, 01.06.2012, p. 231-240.

Research output: Contribution to journalArticle

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AU - Kim, Kwangwook

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AU - Kim, Hwa Jung

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