Myricetin is a biologically active natural polyphenol with beneficial effects on metabolic health. This study aimed to examine the effects of myricetin on the expression levels of genes involved in lipolysis and mitochondrial respiration in adipocytes and the anti-obesity potential of myricetin. The results indicated that myricetin reduced triglyceride (TG) content and increased mitochondrial content and oxygen consumption rate (OCR) in adipocytes in vitro. To determine anti-obesity effect of myricetin, C57BL6/J mice were fed a high-fat diet (HFD) for eight weeks and then treated with myricetin (10 mg/kg) for 2 weeks. The in vivo treatment of myricetin reduced body weight by 11%. Furthermore, it improved the glucose tolerance, and increased fatty acid consumption of HFD-fed mice. Myricetin treatment increased Sirt3 expression and reduced the acetylation of mitochondrial proteins in adipose tissue. Finally, the knockdown of Sirt3 in adipocytes reduced the myricetin-induced increase in mitochondrial oxygen consumption rate by about 27% compared to controls. Our results indicated that myricetin exerted anti-obesity effects through the upregulation of Sirt3 expression and mitochondrial metabolism in adipose tissue.
Bibliographical noteFunding Information:
Funding: This research was supported by National Research Foundation of Korea grant (No. NRF-2014R1A6A3 A04056472 (YHL)), by the Bio & Medical Technology Development Program of the NRF (No. NRF-2016M3A9D5 A01953818), and by Research Resettlement Fund for the new faculty of Seoul National University. This work was performed within the program of the AMOREPACIFIC Open Research ‘ORT19-01-16E705001’ supported by a grant from AMOREPACIFIC.
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All Science Journal Classification (ASJC) codes
- Food Science
- Nutrition and Dietetics