N-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice

Ja Kyung Kim, Soo Min Cho, So Hee Kang, Eunjung Kim, Hee Yi, Eun Sun Yun, Dong Goo Lee, Hee Jung Cho, Yong Han Paik, Yang Kyu Choi, Seung Joo Haam, Ho Chul Shin, Dong Ki Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background and Aim: The increasing prevalence of cholesterol gallstone (CG) disease has become an economic burden to the healthcare system. Ursodeoxycholic acid (UDCA) is the only established medical agent used to dissolve gallstones. In investigating novel therapeutics for CG, we assessed the preventive effects of n-3 polyunsaturated fatty acids (n-3PUFA) on the formation of CG induced by feeding a lithogenic diet (LD) containing high cholesterol levels to mice. Methods: Mice were divided into the following six groups: (A) regular diet (RD); (B) RD+n-3PUFA; (C) LD; (D) LD+n-3PUFA; (E) LD+UDCA; (F) LD+n-3PUFA+UDCA. After RD/LD feeding for 2weeks, n-3PUFA or UDCA was administered orally and the diet maintained for 8weeks. The levels of phospholipids and cholesterol in bile, CG formation, gallbladder wall thickness, MUC gene expression in gallbladder were analyzed. Results: No stone or sludge was evident in the RD groups (Groups A, B). Mice in the n-3PUFA treatment (Groups D, F) showed significantly lower stone formation than the other LD groups (Groups C, E). The combination treatment of n-3PUFA and UDCA suppressed stone formation more than mono-therapy with n-3PUFA or UDCA. Bile phospholipid levels were significantly elevated in the Group F. Hypertrophy of the gallbladder wall was evident in mice fed LD. MUC 2, 5AC, 5B and 6 mRNA expression levels were significantly elevated in the LD-fed group, and this was suppressed by n-3PUFA with or without UDCA. Conclusions: N-3PUFA attenuated gallstone formation in mouse, through increasing the levels of bile phospholipids and suppressing bile mucin formation.

Original languageEnglish
Pages (from-to)1745-1751
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume27
Issue number11
DOIs
Publication statusPublished - 2012 Nov 1

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Omega-3 Fatty Acids
Gallstones
Mucins
Cholesterol
Diet
Ursodeoxycholic Acid
Bile
Gallbladder
Phospholipids
Sewage
Hypercholesterolemia
Hypertrophy

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Kim, Ja Kyung ; Cho, Soo Min ; Kang, So Hee ; Kim, Eunjung ; Yi, Hee ; Yun, Eun Sun ; Lee, Dong Goo ; Cho, Hee Jung ; Paik, Yong Han ; Choi, Yang Kyu ; Haam, Seung Joo ; Shin, Ho Chul ; Lee, Dong Ki. / N-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice. In: Journal of Gastroenterology and Hepatology (Australia). 2012 ; Vol. 27, No. 11. pp. 1745-1751.
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abstract = "Background and Aim: The increasing prevalence of cholesterol gallstone (CG) disease has become an economic burden to the healthcare system. Ursodeoxycholic acid (UDCA) is the only established medical agent used to dissolve gallstones. In investigating novel therapeutics for CG, we assessed the preventive effects of n-3 polyunsaturated fatty acids (n-3PUFA) on the formation of CG induced by feeding a lithogenic diet (LD) containing high cholesterol levels to mice. Methods: Mice were divided into the following six groups: (A) regular diet (RD); (B) RD+n-3PUFA; (C) LD; (D) LD+n-3PUFA; (E) LD+UDCA; (F) LD+n-3PUFA+UDCA. After RD/LD feeding for 2weeks, n-3PUFA or UDCA was administered orally and the diet maintained for 8weeks. The levels of phospholipids and cholesterol in bile, CG formation, gallbladder wall thickness, MUC gene expression in gallbladder were analyzed. Results: No stone or sludge was evident in the RD groups (Groups A, B). Mice in the n-3PUFA treatment (Groups D, F) showed significantly lower stone formation than the other LD groups (Groups C, E). The combination treatment of n-3PUFA and UDCA suppressed stone formation more than mono-therapy with n-3PUFA or UDCA. Bile phospholipid levels were significantly elevated in the Group F. Hypertrophy of the gallbladder wall was evident in mice fed LD. MUC 2, 5AC, 5B and 6 mRNA expression levels were significantly elevated in the LD-fed group, and this was suppressed by n-3PUFA with or without UDCA. Conclusions: N-3PUFA attenuated gallstone formation in mouse, through increasing the levels of bile phospholipids and suppressing bile mucin formation.",
author = "Kim, {Ja Kyung} and Cho, {Soo Min} and Kang, {So Hee} and Eunjung Kim and Hee Yi and Yun, {Eun Sun} and Lee, {Dong Goo} and Cho, {Hee Jung} and Paik, {Yong Han} and Choi, {Yang Kyu} and Haam, {Seung Joo} and Shin, {Ho Chul} and Lee, {Dong Ki}",
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N-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice. / Kim, Ja Kyung; Cho, Soo Min; Kang, So Hee; Kim, Eunjung; Yi, Hee; Yun, Eun Sun; Lee, Dong Goo; Cho, Hee Jung; Paik, Yong Han; Choi, Yang Kyu; Haam, Seung Joo; Shin, Ho Chul; Lee, Dong Ki.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 27, No. 11, 01.11.2012, p. 1745-1751.

Research output: Contribution to journalArticle

TY - JOUR

T1 - N-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice

AU - Kim, Ja Kyung

AU - Cho, Soo Min

AU - Kang, So Hee

AU - Kim, Eunjung

AU - Yi, Hee

AU - Yun, Eun Sun

AU - Lee, Dong Goo

AU - Cho, Hee Jung

AU - Paik, Yong Han

AU - Choi, Yang Kyu

AU - Haam, Seung Joo

AU - Shin, Ho Chul

AU - Lee, Dong Ki

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Background and Aim: The increasing prevalence of cholesterol gallstone (CG) disease has become an economic burden to the healthcare system. Ursodeoxycholic acid (UDCA) is the only established medical agent used to dissolve gallstones. In investigating novel therapeutics for CG, we assessed the preventive effects of n-3 polyunsaturated fatty acids (n-3PUFA) on the formation of CG induced by feeding a lithogenic diet (LD) containing high cholesterol levels to mice. Methods: Mice were divided into the following six groups: (A) regular diet (RD); (B) RD+n-3PUFA; (C) LD; (D) LD+n-3PUFA; (E) LD+UDCA; (F) LD+n-3PUFA+UDCA. After RD/LD feeding for 2weeks, n-3PUFA or UDCA was administered orally and the diet maintained for 8weeks. The levels of phospholipids and cholesterol in bile, CG formation, gallbladder wall thickness, MUC gene expression in gallbladder were analyzed. Results: No stone or sludge was evident in the RD groups (Groups A, B). Mice in the n-3PUFA treatment (Groups D, F) showed significantly lower stone formation than the other LD groups (Groups C, E). The combination treatment of n-3PUFA and UDCA suppressed stone formation more than mono-therapy with n-3PUFA or UDCA. Bile phospholipid levels were significantly elevated in the Group F. Hypertrophy of the gallbladder wall was evident in mice fed LD. MUC 2, 5AC, 5B and 6 mRNA expression levels were significantly elevated in the LD-fed group, and this was suppressed by n-3PUFA with or without UDCA. Conclusions: N-3PUFA attenuated gallstone formation in mouse, through increasing the levels of bile phospholipids and suppressing bile mucin formation.

AB - Background and Aim: The increasing prevalence of cholesterol gallstone (CG) disease has become an economic burden to the healthcare system. Ursodeoxycholic acid (UDCA) is the only established medical agent used to dissolve gallstones. In investigating novel therapeutics for CG, we assessed the preventive effects of n-3 polyunsaturated fatty acids (n-3PUFA) on the formation of CG induced by feeding a lithogenic diet (LD) containing high cholesterol levels to mice. Methods: Mice were divided into the following six groups: (A) regular diet (RD); (B) RD+n-3PUFA; (C) LD; (D) LD+n-3PUFA; (E) LD+UDCA; (F) LD+n-3PUFA+UDCA. After RD/LD feeding for 2weeks, n-3PUFA or UDCA was administered orally and the diet maintained for 8weeks. The levels of phospholipids and cholesterol in bile, CG formation, gallbladder wall thickness, MUC gene expression in gallbladder were analyzed. Results: No stone or sludge was evident in the RD groups (Groups A, B). Mice in the n-3PUFA treatment (Groups D, F) showed significantly lower stone formation than the other LD groups (Groups C, E). The combination treatment of n-3PUFA and UDCA suppressed stone formation more than mono-therapy with n-3PUFA or UDCA. Bile phospholipid levels were significantly elevated in the Group F. Hypertrophy of the gallbladder wall was evident in mice fed LD. MUC 2, 5AC, 5B and 6 mRNA expression levels were significantly elevated in the LD-fed group, and this was suppressed by n-3PUFA with or without UDCA. Conclusions: N-3PUFA attenuated gallstone formation in mouse, through increasing the levels of bile phospholipids and suppressing bile mucin formation.

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