N-acetylglucosaminidase activities in wetlands

A global survey

Hojeong Kang, Chris Freeman, Soon Park Seok, Jongsik Chun

Research output: Contribution to journalReview article

27 Citations (Scopus)

Abstract

A fluorogenic model substrate, methylumbelliferyl-N-acetylglucosamine was used to measure N-acetylglucosaminidase activities in wetland soils. The enzyme activity exhibited substrate saturation around 200 μM, and an approximate linearity for at least 90 min incubation time. The effects of temperature, pH, toluene, and γ-radiation on the enzyme were also determined. The method was applied to 32 different wetland samples from both freshwater wetlands and salt-water wetlands. Overall, the activities were the lowest in bogs and much higher in freshwater marshes and flooded grasslands. The variations of the activity were not explained by a single environmental variable. However, among peatland samples (e.g., bogs and fens) only, specific enzyme activity per organic matter exhibited a significant correlation with N-acetylglucosaminidase activity, suggesting the importance of the enzyme activity in organic matter dynamics in such systems.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalHydrobiologia
Volume532
Issue number1
DOIs
Publication statusPublished - 2005 Jan 1

Fingerprint

wetlands
wetland
bogs
enzyme activity
bog
organic matter
wetland soils
N-acetylglucosamine
toluene
fens
peatlands
saline water
substrate
marshes
fen
salt water
linearity
grasslands
peatland
marsh

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Kang, Hojeong ; Freeman, Chris ; Seok, Soon Park ; Chun, Jongsik. / N-acetylglucosaminidase activities in wetlands : A global survey. In: Hydrobiologia. 2005 ; Vol. 532, No. 1. pp. 103-110.
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N-acetylglucosaminidase activities in wetlands : A global survey. / Kang, Hojeong; Freeman, Chris; Seok, Soon Park; Chun, Jongsik.

In: Hydrobiologia, Vol. 532, No. 1, 01.01.2005, p. 103-110.

Research output: Contribution to journalReview article

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AB - A fluorogenic model substrate, methylumbelliferyl-N-acetylglucosamine was used to measure N-acetylglucosaminidase activities in wetland soils. The enzyme activity exhibited substrate saturation around 200 μM, and an approximate linearity for at least 90 min incubation time. The effects of temperature, pH, toluene, and γ-radiation on the enzyme were also determined. The method was applied to 32 different wetland samples from both freshwater wetlands and salt-water wetlands. Overall, the activities were the lowest in bogs and much higher in freshwater marshes and flooded grasslands. The variations of the activity were not explained by a single environmental variable. However, among peatland samples (e.g., bogs and fens) only, specific enzyme activity per organic matter exhibited a significant correlation with N-acetylglucosaminidase activity, suggesting the importance of the enzyme activity in organic matter dynamics in such systems.

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