Na+/Ca2+ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia

In Koo Hwang; Ki Yeon Yoo; Dae Won Kim; Tae Cheon Kang; Soo Young Choi; Young Guen Kwon; Byoung Hee Han; Jong Sung Kim; Moo Ho Won

doi:10.1016/j.brainres.2006.02.048

Na⁺/Ca²⁺ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia

In Koo Hwang, Ki Yeon Yoo, Dae Won Kim, Tae Cheon Kang, Soo Young Choi, Young Guen Kwon, Byoung Hee Han, Jong Sung Kim, Moo Ho Won

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Alterations of immunoreactivity and protein contents of Na⁺/Ca²⁺ exchanger 1 (NCX1) were observed in the gerbil hippocampus proper after 5 min of transient forebrain ischemia. NCX1 immunoreactivity was significantly changed in the hippocampal CA1 region, but not in the CA2/3 region after ischemia/reperfusion. In the sham-operated group, NCX1 immunoreactivity was mainly detected in CA1 pyramidal cells. However, 30 min after ischemia/reperfusion, NCX1 immunoreactivity was significantly decreased and then increased at 1 day after ischemia. At this time, NCX1 immunoreactivity in CA1 pyramidal cells was similar to that of the sham-operated group. At 3 days after ischemia, NCX1 immunoreactivity was significantly reduced in the CA1 region compared to that of the sham-operated group and NCX1 immunoreactivity was significantly increased again 4 days after ischemia. Thereafter, NCX1 immunoreactivity was decreased time-dependently in ischemia groups. Between 15 min and 6 h post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum of the CA1 region. From 3 days post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum. Ischemia-induced changes in NCX1 protein contents in the hippocampus proper concurred with immunohistochemical data post-ischemia. Our results suggest that changes in NCX1 in CA1 pyramidal cells and astrocytes after ischemia are associated with intracellular Na⁺ concentrations and that NCX1 may induce an intracellular calcium overload, which may be related to neuronal death.

Original language	English
Pages (from-to)	181-190
Number of pages	10
Journal	Brain Research
Volume	1086
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2006.02.048
Publication status	Published - 2006 May 1

Bibliographical note

Funding Information:
The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee, and Ms. Hyun Sook Kim for their technical help for this study. This study was supported by a grant of the Korean Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A030079).

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2006.02.048

Cite this

@article{bb7af46449734978b389600042936523,

title = "Na+/Ca2+ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia",

abstract = "Alterations of immunoreactivity and protein contents of Na+/Ca2+ exchanger 1 (NCX1) were observed in the gerbil hippocampus proper after 5 min of transient forebrain ischemia. NCX1 immunoreactivity was significantly changed in the hippocampal CA1 region, but not in the CA2/3 region after ischemia/reperfusion. In the sham-operated group, NCX1 immunoreactivity was mainly detected in CA1 pyramidal cells. However, 30 min after ischemia/reperfusion, NCX1 immunoreactivity was significantly decreased and then increased at 1 day after ischemia. At this time, NCX1 immunoreactivity in CA1 pyramidal cells was similar to that of the sham-operated group. At 3 days after ischemia, NCX1 immunoreactivity was significantly reduced in the CA1 region compared to that of the sham-operated group and NCX1 immunoreactivity was significantly increased again 4 days after ischemia. Thereafter, NCX1 immunoreactivity was decreased time-dependently in ischemia groups. Between 15 min and 6 h post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum of the CA1 region. From 3 days post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum. Ischemia-induced changes in NCX1 protein contents in the hippocampus proper concurred with immunohistochemical data post-ischemia. Our results suggest that changes in NCX1 in CA1 pyramidal cells and astrocytes after ischemia are associated with intracellular Na+ concentrations and that NCX1 may induce an intracellular calcium overload, which may be related to neuronal death.",

author = "{Koo Hwang}, In and Yoo, {Ki Yeon} and {Won Kim}, Dae and Kang, {Tae Cheon} and {Young Choi}, Soo and Kwon, {Young Guen} and {Hee Han}, Byoung and {Sung Kim}, Jong and Won, {Moo Ho}",

note = "Funding Information: The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee, and Ms. Hyun Sook Kim for their technical help for this study. This study was supported by a grant of the Korean Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A030079).",

year = "2006",

month = may,

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doi = "10.1016/j.brainres.2006.02.048",

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journal = "Brain Research",

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Na⁺/Ca²⁺ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia. / Koo Hwang, In; Yoo, Ki Yeon; Won Kim, Dae; Kang, Tae Cheon; Young Choi, Soo; Kwon, Young Guen; Hee Han, Byoung; Sung Kim, Jong; Won, Moo Ho.

In: Brain Research, Vol. 1086, No. 1, 01.05.2006, p. 181-190.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Na+/Ca2+ exchanger 1 alters in pyramidal cells and expresses in astrocytes of the gerbil hippocampal CA1 region after ischemia

AU - Koo Hwang, In

AU - Yoo, Ki Yeon

AU - Won Kim, Dae

AU - Kang, Tae Cheon

AU - Young Choi, Soo

AU - Kwon, Young Guen

AU - Hee Han, Byoung

AU - Sung Kim, Jong

AU - Won, Moo Ho

N1 - Funding Information: The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee, and Ms. Hyun Sook Kim for their technical help for this study. This study was supported by a grant of the Korean Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A030079).

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Alterations of immunoreactivity and protein contents of Na+/Ca2+ exchanger 1 (NCX1) were observed in the gerbil hippocampus proper after 5 min of transient forebrain ischemia. NCX1 immunoreactivity was significantly changed in the hippocampal CA1 region, but not in the CA2/3 region after ischemia/reperfusion. In the sham-operated group, NCX1 immunoreactivity was mainly detected in CA1 pyramidal cells. However, 30 min after ischemia/reperfusion, NCX1 immunoreactivity was significantly decreased and then increased at 1 day after ischemia. At this time, NCX1 immunoreactivity in CA1 pyramidal cells was similar to that of the sham-operated group. At 3 days after ischemia, NCX1 immunoreactivity was significantly reduced in the CA1 region compared to that of the sham-operated group and NCX1 immunoreactivity was significantly increased again 4 days after ischemia. Thereafter, NCX1 immunoreactivity was decreased time-dependently in ischemia groups. Between 15 min and 6 h post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum of the CA1 region. From 3 days post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum. Ischemia-induced changes in NCX1 protein contents in the hippocampus proper concurred with immunohistochemical data post-ischemia. Our results suggest that changes in NCX1 in CA1 pyramidal cells and astrocytes after ischemia are associated with intracellular Na+ concentrations and that NCX1 may induce an intracellular calcium overload, which may be related to neuronal death.

AB - Alterations of immunoreactivity and protein contents of Na+/Ca2+ exchanger 1 (NCX1) were observed in the gerbil hippocampus proper after 5 min of transient forebrain ischemia. NCX1 immunoreactivity was significantly changed in the hippocampal CA1 region, but not in the CA2/3 region after ischemia/reperfusion. In the sham-operated group, NCX1 immunoreactivity was mainly detected in CA1 pyramidal cells. However, 30 min after ischemia/reperfusion, NCX1 immunoreactivity was significantly decreased and then increased at 1 day after ischemia. At this time, NCX1 immunoreactivity in CA1 pyramidal cells was similar to that of the sham-operated group. At 3 days after ischemia, NCX1 immunoreactivity was significantly reduced in the CA1 region compared to that of the sham-operated group and NCX1 immunoreactivity was significantly increased again 4 days after ischemia. Thereafter, NCX1 immunoreactivity was decreased time-dependently in ischemia groups. Between 15 min and 6 h post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum of the CA1 region. From 3 days post-ischemia, NCX1 immunoreactivity was expressed in astrocytes in the strata oriens and radiatum. Ischemia-induced changes in NCX1 protein contents in the hippocampus proper concurred with immunohistochemical data post-ischemia. Our results suggest that changes in NCX1 in CA1 pyramidal cells and astrocytes after ischemia are associated with intracellular Na+ concentrations and that NCX1 may induce an intracellular calcium overload, which may be related to neuronal death.

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