We demonstrate the fabrication of nano-modified collagen hydrogels that afford controlled growth factor release for induced pluripotent stem (iPS) cell culture. Specifically, we focused on the preparation and characterization of graphene oxide (GO) sheets incorporated into collagen-based hydrogels for controlled growth factor release. Fibroblast growth factor-2 (FGF-2) bound to collagen via electrostatic interactions and partial hydrogen bonding. Various GO sheet quantities were incorporated and evenly distributed in collagen hydrogels. Taking advantage of the low permeability of GO sheets, FGF-2 release was regulated in a controlled manner. In this manuscript, three different GO sheet concentrations were applied to obtain different release profiles over a period of 400. h in the presence of serum. The incorporation of GO sheets into collagen hydrogels offers a new approach to control the release of biologically active macromolecules (e.g., growth factors).
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning of Korea government 2012M3A9C6050104 , 2014K2A7A1044460 and 2013R1A1A1076126 . Additionally, this research was also supported by a Grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea ( HI14C-3266–030014 ). This work was also supported by a Grant from the Next-Generation BioGreen 21 Program (No. PJ009986012015 ) (Rural Development Administration, Republic of Korea). In addition, This work was supported by a Grant from Kyung Hee University in 2014 ( KHU-20140762 ).
© 2015 Elsevier Ltd.
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)
- Industrial and Manufacturing Engineering