Nano-formulation of a photosensitizer using a DNA tetrahedron and its potential for in vivo photodynamic therapy

Kyoung Ran Kim, Duhee Bang, Dae Ro Ahn

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Photodynamic therapy (PDT) is a cytotoxic treatment using singlet oxygen produced by photosensitizers. Approved porphyrinoid PDT still suffers from a lack of robust production methods and low water solubility. Methylene blue (MB) is a good candidate for the PDT drug, because the dye is an effective photosensitizer, can be easily synthesized, and is already being used in other clinical fields. However, its poor cell/tissue penetration and low stability against the reducible biological conditions should be addressed by using a proper delivery vehicle. Here, we employed a DNA tetrahedron, a self-assembled nanostructure as the carrier for intracellular delivery of MB by taking advantage of the DNA binding property of the photosensitizer and demonstrated photo-induced cytotoxicity by the MB delivered by the DNA nanocarrier. We also evaluated the PDT potency of the MB-loaded DNA nanoconstruct in vivo tumor model to suppress tumor growth.

Original languageEnglish
Pages (from-to)605-609
Number of pages5
JournalBiomaterials Science
Volume4
Issue number4
DOIs
Publication statusPublished - 2016 Apr

Bibliographical note

Funding Information:
This study was supported by the intramural programs of KIST, the Global Innovative Research Center program (2012K1A1A2A01055811) of the National Research Foundation of Korea and by the Intramural Research Programs (Global RNAi Carrier Initiative and Convergence Technology Program) of KIST, the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2014-R1A2A2A04002526), and a grant by the Pioneer Research Center Program through NRF funded by the Ministry of Science, ICT and Future Planning (2014M3C1A3054141).

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)

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