Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body

Uiyoung Han; Wijin Kim; Hyeonjin Cha; Ju Hyun Park; Jinkee Hong

doi:10.1016/j.isci.2021.102297

Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body

Uiyoung Han, Wijin Kim, Hyeonjin Cha, Ju Hyun Park, Jinkee Hong

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Individual cell environment stimulating single cell is a suitable strategy for the generation of sophisticated multicellular aggregates with localized biochemical signaling. However, such strategy for induced pluripotent stem cell (iPSC)-derived embryoid bodies (EBs) is limited because the presence of external stimulation can inhibit spontaneous cellular communication, resulting in misdirection in the maturation and differentiation of EBs. In this study, a facile method of engineering the iPSC membrane to stimulate the inner cell of EBs while maintaining cellular activities is reported. We coated the iPSC surface with nanoscale extracellular matrix fabricated by self-assembly between vitronectin and heparin. This nano-coating allowed iPSC to retain its in vitro properties including adhesion capability, proliferation, and pluripotency during its aggregation. More importantly, the nano-coating did not induce lineage-specific differentiation but increased E-cadherin expression, resulting in promotion of development of EB. This study provides a foundation for future production of sophisticated patient-specific multicellular aggregates by modification of living cell membranes.

Original language	English
Article number	102297
Journal	iScience
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.isci.2021.102297
Publication status	Published - 2021 Apr 23

Bibliographical note

Funding Information:
This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2016M3A9C6917405, 2016M3A9C6917402, and 2019M3A9H110378611). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343 and 2020R1A4A1016093). U.H. W.K. J.H.P. and J.H. conceived the study; U.H. W.K. and H.C. performed research; U.H. W.K. H.C. J.H.P. and J.H. analyzed data; U.H. and W.K. wrote the manuscript; and J.H.P. and J.H. edited the manuscript. The authors declare no competing interests.

Funding Information:
This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2016M3A9C6917405 , 2016M3A9C6917402 , and 2019M3A9H110378611 ). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF- 2017R1E1A1A01074343 and 2020R1A4A1016093 ).

Publisher Copyright:
© 2021 The Authors

All Science Journal Classification (ASJC) codes

General

Access to Document

10.1016/j.isci.2021.102297

Cite this

@article{bcf254b043b746f0925e5b88a1835a9c,

title = "Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body",

abstract = "Individual cell environment stimulating single cell is a suitable strategy for the generation of sophisticated multicellular aggregates with localized biochemical signaling. However, such strategy for induced pluripotent stem cell (iPSC)-derived embryoid bodies (EBs) is limited because the presence of external stimulation can inhibit spontaneous cellular communication, resulting in misdirection in the maturation and differentiation of EBs. In this study, a facile method of engineering the iPSC membrane to stimulate the inner cell of EBs while maintaining cellular activities is reported. We coated the iPSC surface with nanoscale extracellular matrix fabricated by self-assembly between vitronectin and heparin. This nano-coating allowed iPSC to retain its in vitro properties including adhesion capability, proliferation, and pluripotency during its aggregation. More importantly, the nano-coating did not induce lineage-specific differentiation but increased E-cadherin expression, resulting in promotion of development of EB. This study provides a foundation for future production of sophisticated patient-specific multicellular aggregates by modification of living cell membranes.",

author = "Uiyoung Han and Wijin Kim and Hyeonjin Cha and Park, {Ju Hyun} and Jinkee Hong",

note = "Funding Information: This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2016M3A9C6917405, 2016M3A9C6917402, and 2019M3A9H110378611). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343 and 2020R1A4A1016093). U.H. W.K. J.H.P. and J.H. conceived the study; U.H. W.K. and H.C. performed research; U.H. W.K. H.C. J.H.P. and J.H. analyzed data; U.H. and W.K. wrote the manuscript; and J.H.P. and J.H. edited the manuscript. The authors declare no competing interests. Funding Information: This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2016M3A9C6917405 , 2016M3A9C6917402 , and 2019M3A9H110378611 ). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF- 2017R1E1A1A01074343 and 2020R1A4A1016093 ). Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = apr,

day = "23",

doi = "10.1016/j.isci.2021.102297",

language = "English",

volume = "24",

journal = "iScience",

issn = "2589-0042",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body

AU - Han, Uiyoung

AU - Kim, Wijin

AU - Cha, Hyeonjin

AU - Park, Ju Hyun

AU - Hong, Jinkee

N1 - Funding Information: This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2016M3A9C6917405, 2016M3A9C6917402, and 2019M3A9H110378611). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343 and 2020R1A4A1016093). U.H. W.K. J.H.P. and J.H. conceived the study; U.H. W.K. and H.C. performed research; U.H. W.K. H.C. J.H.P. and J.H. analyzed data; U.H. and W.K. wrote the manuscript; and J.H.P. and J.H. edited the manuscript. The authors declare no competing interests. Funding Information: This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2016M3A9C6917405 , 2016M3A9C6917402 , and 2019M3A9H110378611 ). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF- 2017R1E1A1A01074343 and 2020R1A4A1016093 ). Publisher Copyright: © 2021 The Authors

PY - 2021/4/23

Y1 - 2021/4/23

N2 - Individual cell environment stimulating single cell is a suitable strategy for the generation of sophisticated multicellular aggregates with localized biochemical signaling. However, such strategy for induced pluripotent stem cell (iPSC)-derived embryoid bodies (EBs) is limited because the presence of external stimulation can inhibit spontaneous cellular communication, resulting in misdirection in the maturation and differentiation of EBs. In this study, a facile method of engineering the iPSC membrane to stimulate the inner cell of EBs while maintaining cellular activities is reported. We coated the iPSC surface with nanoscale extracellular matrix fabricated by self-assembly between vitronectin and heparin. This nano-coating allowed iPSC to retain its in vitro properties including adhesion capability, proliferation, and pluripotency during its aggregation. More importantly, the nano-coating did not induce lineage-specific differentiation but increased E-cadherin expression, resulting in promotion of development of EB. This study provides a foundation for future production of sophisticated patient-specific multicellular aggregates by modification of living cell membranes.

AB - Individual cell environment stimulating single cell is a suitable strategy for the generation of sophisticated multicellular aggregates with localized biochemical signaling. However, such strategy for induced pluripotent stem cell (iPSC)-derived embryoid bodies (EBs) is limited because the presence of external stimulation can inhibit spontaneous cellular communication, resulting in misdirection in the maturation and differentiation of EBs. In this study, a facile method of engineering the iPSC membrane to stimulate the inner cell of EBs while maintaining cellular activities is reported. We coated the iPSC surface with nanoscale extracellular matrix fabricated by self-assembly between vitronectin and heparin. This nano-coating allowed iPSC to retain its in vitro properties including adhesion capability, proliferation, and pluripotency during its aggregation. More importantly, the nano-coating did not induce lineage-specific differentiation but increased E-cadherin expression, resulting in promotion of development of EB. This study provides a foundation for future production of sophisticated patient-specific multicellular aggregates by modification of living cell membranes.

UR - http://www.scopus.com/inward/record.url?scp=85103310655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85103310655&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2021.102297

DO - 10.1016/j.isci.2021.102297

M3 - Article

AN - SCOPUS:85103310655

SN - 2589-0042

VL - 24

JO - iScience

JF - iScience

IS - 4

M1 - 102297

ER -

Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this