Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body

Uiyoung Han, Wijin Kim, Hyeonjin Cha, Ju Hyun Park, Jinkee Hong

Research output: Contribution to journalArticlepeer-review

Abstract

Individual cell environment stimulating single cell is a suitable strategy for the generation of sophisticated multicellular aggregates with localized biochemical signaling. However, such strategy for induced pluripotent stem cell (iPSC)-derived embryoid bodies (EBs) is limited because the presence of external stimulation can inhibit spontaneous cellular communication, resulting in misdirection in the maturation and differentiation of EBs. In this study, a facile method of engineering the iPSC membrane to stimulate the inner cell of EBs while maintaining cellular activities is reported. We coated the iPSC surface with nanoscale extracellular matrix fabricated by self-assembly between vitronectin and heparin. This nano-coating allowed iPSC to retain its in vitro properties including adhesion capability, proliferation, and pluripotency during its aggregation. More importantly, the nano-coating did not induce lineage-specific differentiation but increased E-cadherin expression, resulting in promotion of development of EB. This study provides a foundation for future production of sophisticated patient-specific multicellular aggregates by modification of living cell membranes.

Original languageEnglish
Article number102297
JournaliScience
Volume24
Issue number4
DOIs
Publication statusPublished - 2021 Apr 23

Bibliographical note

Funding Information:
This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2016M3A9C6917405, 2016M3A9C6917402, and 2019M3A9H110378611). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343 and 2020R1A4A1016093). U.H. W.K. J.H.P. and J.H. conceived the study; U.H. W.K. and H.C. performed research; U.H. W.K. H.C. J.H.P. and J.H. analyzed data; U.H. and W.K. wrote the manuscript; and J.H.P. and J.H. edited the manuscript. The authors declare no competing interests.

Funding Information:
This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2016M3A9C6917405 , 2016M3A9C6917402 , and 2019M3A9H110378611 ). Additionally, this research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF- 2017R1E1A1A01074343 and 2020R1A4A1016093 ).

Publisher Copyright:
© 2021 The Authors

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Nano-structure of vitronectin/heparin on cell membrane for stimulating single cell in iPSC-derived embryoid body'. Together they form a unique fingerprint.

Cite this