Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis

Gayoung Eom, Hongki Kim, Ahreum Hwang, Hye Young Son, Yuna Choi, Jeong Moon, Donghyeong Kim, Miyeon Lee, Eun Kyung Lim, Jinyoung Jeong, Yong Min Huh, Min Kyo Seo, Taejoon Kang, Bongsoo Kim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nanoparticles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL−1. Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor-bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing.

Original languageEnglish
Article number1701832
JournalAdvanced Functional Materials
Volume27
Issue number37
DOIs
Publication statusPublished - 2017 Oct 5

Fingerprint

Telomerase
breast
Nanowires
Raman scattering
nanowires
cancer
Tissue
Raman spectra
Cells
sensors
Sensors
Tumors
Bearings (structural)
primers
tumors
biomarkers
Biomarkers
Substrates
Intercalation
Elongation

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrochemistry

Cite this

Eom, Gayoung ; Kim, Hongki ; Hwang, Ahreum ; Son, Hye Young ; Choi, Yuna ; Moon, Jeong ; Kim, Donghyeong ; Lee, Miyeon ; Lim, Eun Kyung ; Jeong, Jinyoung ; Huh, Yong Min ; Seo, Min Kyo ; Kang, Taejoon ; Kim, Bongsoo. / Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection : Application to Gastric and Breast Cancer Tissues Diagnosis. In: Advanced Functional Materials. 2017 ; Vol. 27, No. 37.
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abstract = "Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85{\%} of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nanoparticles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL−1. Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor-bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing.",
author = "Gayoung Eom and Hongki Kim and Ahreum Hwang and Son, {Hye Young} and Yuna Choi and Jeong Moon and Donghyeong Kim and Miyeon Lee and Lim, {Eun Kyung} and Jinyoung Jeong and Huh, {Yong Min} and Seo, {Min Kyo} and Taejoon Kang and Bongsoo Kim",
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Eom, G, Kim, H, Hwang, A, Son, HY, Choi, Y, Moon, J, Kim, D, Lee, M, Lim, EK, Jeong, J, Huh, YM, Seo, MK, Kang, T & Kim, B 2017, 'Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis', Advanced Functional Materials, vol. 27, no. 37, 1701832. https://doi.org/10.1002/adfm.201701832

Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection : Application to Gastric and Breast Cancer Tissues Diagnosis. / Eom, Gayoung; Kim, Hongki; Hwang, Ahreum; Son, Hye Young; Choi, Yuna; Moon, Jeong; Kim, Donghyeong; Lee, Miyeon; Lim, Eun Kyung; Jeong, Jinyoung; Huh, Yong Min; Seo, Min Kyo; Kang, Taejoon; Kim, Bongsoo.

In: Advanced Functional Materials, Vol. 27, No. 37, 1701832, 05.10.2017.

Research output: Contribution to journalArticle

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T1 - Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection

T2 - Application to Gastric and Breast Cancer Tissues Diagnosis

AU - Eom, Gayoung

AU - Kim, Hongki

AU - Hwang, Ahreum

AU - Son, Hye Young

AU - Choi, Yuna

AU - Moon, Jeong

AU - Kim, Donghyeong

AU - Lee, Miyeon

AU - Lim, Eun Kyung

AU - Jeong, Jinyoung

AU - Huh, Yong Min

AU - Seo, Min Kyo

AU - Kang, Taejoon

AU - Kim, Bongsoo

PY - 2017/10/5

Y1 - 2017/10/5

N2 - Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nanoparticles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL−1. Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor-bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing.

AB - Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nanoparticles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL−1. Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor-bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing.

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