Nanograting-based plasmon enhancement for total internal reflection fluorescence microscopy of live cells

Kyujung Kim, Dong Jun Kim, Eun Jin Cho, Jin Suck Suh, Yong Min Huh, Donghyun Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We investigated evanescent field enhancement based on subwavelength nanogratings for improved sensitivity in total internal reflection microscopy of live cells. The field enhancement is associated with subwavelength-grating- coupled plasmon excitation. An optimum sample employed a silver grating on a silver film and an SF10 glass substrate. Field intensity was enhanced by approximately 90% when measured by fluorescent excitation of microbeads relative to that on a bare prism as a control, which is in good agreement with numerical results. The subwavelength-grating-mediated field enhancement was also applied to live cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively.

Original languageEnglish
Article number015202
JournalNanotechnology
Volume20
Issue number1
DOIs
Publication statusPublished - 2009 Jan 7

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Fluorescence microscopy
Silver
Evanescent fields
Prisms
Semiconductor quantum dots
Microscopic examination
Imaging techniques
Glass
Substrates

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

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abstract = "We investigated evanescent field enhancement based on subwavelength nanogratings for improved sensitivity in total internal reflection microscopy of live cells. The field enhancement is associated with subwavelength-grating- coupled plasmon excitation. An optimum sample employed a silver grating on a silver film and an SF10 glass substrate. Field intensity was enhanced by approximately 90{\%} when measured by fluorescent excitation of microbeads relative to that on a bare prism as a control, which is in good agreement with numerical results. The subwavelength-grating-mediated field enhancement was also applied to live cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively.",
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Nanograting-based plasmon enhancement for total internal reflection fluorescence microscopy of live cells. / Kim, Kyujung; Kim, Dong Jun; Cho, Eun Jin; Suh, Jin Suck; Huh, Yong Min; Kim, Donghyun.

In: Nanotechnology, Vol. 20, No. 1, 015202, 07.01.2009.

Research output: Contribution to journalArticle

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AU - Kim, Donghyun

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