Nanomagnetic System for Rapid Diagnosis of Acute Infection

Ki Soo Park, Hoyoung Kim, Soojin Kim, Kyungheon Lee, Sohyeon Park, Jun Song, Changwook Min, Farhana Khanam, Rasheduzzaman Rashu, Taufiqur Rahman Bhuiyan, Edward T. Ryan, Firdausi Qadri, Ralph Weissleder, Jinwoo Cheon, Richelle C. Charles, Hakho Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pathogen-activated antibody-secreting cells (ASCs) produce and secrete antigen-specific antibodies. ASCs are detectable in the peripheral blood as early as 3 days after antigen exposure, which makes ASCs a potential biomarker for early disease detection. Here, we present a magnetic capture and detection (MCD) assay for sensitive, on-site detection of ASCs. In this approach, ASCs are enriched through magnetic capture, and secreted antibodies are magnetically detected by a miniaturized nuclear magnetic resonance (μNMR) system. This approach is based entirely on magnetics, which supports high contrast against biological background and simplifies assay procedures. We advanced the MCD system by (i) synthesizing magnetic nanoparticles with high magnetic moments for both cell capture and antibody detection, (ii) developing a miniaturized magnetic device for high-yield cell capture, and (iii) optimizing the μNMR assay for antibody detection. Antibody responses targeting hemolysin E (HlyE) can accurately identify individuals with acute enteric fever. As a proof-of-concept, we applied MCD to detect antibodies produced by HlyE-specific hybridoma cells. The MCD achieved high sensitivity in detecting antibodies secreted from as few as 5 hybridoma cells (50 cells/mL). Importantly, the assay could be performed with whole blood with minimal sample processing.

Original languageEnglish
Pages (from-to)11425-11432
Number of pages8
JournalACS Nano
Volume11
Issue number11
DOIs
Publication statusPublished - 2017 Nov 28

Fingerprint

Nanomagnetics
infectious diseases
antibodies
Antibodies
cells
Assays
Hemolysin Proteins
antigens
Antigens
blood
Blood
Nuclear magnetic resonance
fever
Magnetic devices
nuclear magnetic resonance
pathogens
biomarkers
Biomarkers
Pathogens
Magnetic moments

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Park, K. S., Kim, H., Kim, S., Lee, K., Park, S., Song, J., ... Lee, H. (2017). Nanomagnetic System for Rapid Diagnosis of Acute Infection. ACS Nano, 11(11), 11425-11432. https://doi.org/10.1021/acsnano.7b06074
Park, Ki Soo ; Kim, Hoyoung ; Kim, Soojin ; Lee, Kyungheon ; Park, Sohyeon ; Song, Jun ; Min, Changwook ; Khanam, Farhana ; Rashu, Rasheduzzaman ; Bhuiyan, Taufiqur Rahman ; Ryan, Edward T. ; Qadri, Firdausi ; Weissleder, Ralph ; Cheon, Jinwoo ; Charles, Richelle C. ; Lee, Hakho. / Nanomagnetic System for Rapid Diagnosis of Acute Infection. In: ACS Nano. 2017 ; Vol. 11, No. 11. pp. 11425-11432.
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Park, KS, Kim, H, Kim, S, Lee, K, Park, S, Song, J, Min, C, Khanam, F, Rashu, R, Bhuiyan, TR, Ryan, ET, Qadri, F, Weissleder, R, Cheon, J, Charles, RC & Lee, H 2017, 'Nanomagnetic System for Rapid Diagnosis of Acute Infection', ACS Nano, vol. 11, no. 11, pp. 11425-11432. https://doi.org/10.1021/acsnano.7b06074

Nanomagnetic System for Rapid Diagnosis of Acute Infection. / Park, Ki Soo; Kim, Hoyoung; Kim, Soojin; Lee, Kyungheon; Park, Sohyeon; Song, Jun; Min, Changwook; Khanam, Farhana; Rashu, Rasheduzzaman; Bhuiyan, Taufiqur Rahman; Ryan, Edward T.; Qadri, Firdausi; Weissleder, Ralph; Cheon, Jinwoo; Charles, Richelle C.; Lee, Hakho.

In: ACS Nano, Vol. 11, No. 11, 28.11.2017, p. 11425-11432.

Research output: Contribution to journalArticle

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AU - Park, Ki Soo

AU - Kim, Hoyoung

AU - Kim, Soojin

AU - Lee, Kyungheon

AU - Park, Sohyeon

AU - Song, Jun

AU - Min, Changwook

AU - Khanam, Farhana

AU - Rashu, Rasheduzzaman

AU - Bhuiyan, Taufiqur Rahman

AU - Ryan, Edward T.

AU - Qadri, Firdausi

AU - Weissleder, Ralph

AU - Cheon, Jinwoo

AU - Charles, Richelle C.

AU - Lee, Hakho

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Park KS, Kim H, Kim S, Lee K, Park S, Song J et al. Nanomagnetic System for Rapid Diagnosis of Acute Infection. ACS Nano. 2017 Nov 28;11(11):11425-11432. https://doi.org/10.1021/acsnano.7b06074