Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors

Rohit Karnik, Seungpyo Hong, Huanan Zhang, Ying Mei, Daniel G. Anderson, Jeffrey M. Karp, Robert Langer

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We envisioned that label-free control of the transport of cells in two dimensions through receptor-ligand interactions would enable simple separation systems that are easy to implement yet retain the specificity of receptor-ligand interactions. Here we demonstrate nanomechanical control of cell transport in two dimensions via transient receptor-ligand adhesive bonds by patterning of receptors that direct cell rolling through an edge effect HL-60 cells rolling on P-selectin receptor patterns were deflected at angles of 5-10° with respect to their direction of travel. Absence of this effect in the case of rigid microsphere models of cell rolling suggests that this two-dimensional motion depends on nanomechanical properties of the rolling cell. This work suggests the feasibility of simple continuous-flow microfluidic cell separation systems that minimize processing steps and yet retain the specificity of receptor-ligand interactions.

Original languageEnglish
Pages (from-to)1153-1158
Number of pages6
JournalNano letters
Volume8
Issue number4
DOIs
Publication statusPublished - 2008 Apr 1

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Ligands
cells
ligands
P-Selectin
Microspheres
Microfluidics
Labels
Adhesives
interactions
adhesives
travel
Processing

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Karnik, R., Hong, S., Zhang, H., Mei, Y., Anderson, D. G., Karp, J. M., & Langer, R. (2008). Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors. Nano letters, 8(4), 1153-1158. https://doi.org/10.1021/nl073322a
Karnik, Rohit ; Hong, Seungpyo ; Zhang, Huanan ; Mei, Ying ; Anderson, Daniel G. ; Karp, Jeffrey M. ; Langer, Robert. / Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors. In: Nano letters. 2008 ; Vol. 8, No. 4. pp. 1153-1158.
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Karnik, R, Hong, S, Zhang, H, Mei, Y, Anderson, DG, Karp, JM & Langer, R 2008, 'Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors', Nano letters, vol. 8, no. 4, pp. 1153-1158. https://doi.org/10.1021/nl073322a

Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors. / Karnik, Rohit; Hong, Seungpyo; Zhang, Huanan; Mei, Ying; Anderson, Daniel G.; Karp, Jeffrey M.; Langer, Robert.

In: Nano letters, Vol. 8, No. 4, 01.04.2008, p. 1153-1158.

Research output: Contribution to journalArticle

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AU - Karnik, Rohit

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