Nanomechanical in situ monitoring of proteolysis of peptide by cathepsin B

Taeyun Kwon, Jinsung Park, Jaemoon Yang, Dae Sung Yoon, Sungsoo Na, Chang Wan Kim, Jin Suck Suh, Yong Min Huh, Sungjoo Haam, Kilho Eom

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics.

Original languageEnglish
Article numbere6248
JournalPloS one
Volume4
Issue number7
DOIs
Publication statusPublished - 2009 Jul 16

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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