Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

Mostafa Kamal Masud, Jongbeom Na, Tzu En Lin, Victor Malgras, Anant Preet, Abu Ali Ibn Sina, Kathleen Wood, Mutasim Billah, Jeonghun Kim, Jungmok You, Kenya Kani, Andrew E. Whitten, Carlos Salomon, Nam Trung Nguyen, Muhammad J.A. Shiddiky, Matt Trau, Md Shahriar A. Hossain, Yusuke Yamauchi

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)6]3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)6]4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.

Original languageEnglish
Article number112429
JournalBiosensors and Bioelectronics
Volume168
DOIs
Publication statusPublished - 2020 Nov 15

Bibliographical note

Funding Information:
This work is supported by the Australian Research Council ( ARC ) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council ( ARC ) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland ) to M. K. M. C. S. is supported by Lions Medical Research Foundation , National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Científico y Tecnológico ( FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance ( AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland.

Funding Information:
This work is supported by the Australian Research Council (ARC) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council (ARC) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland) to M. K. M. C. S. is supported by Lions Medical Research Foundation, National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico (FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance (AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland.

Publisher Copyright:
© 2020 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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