Nanostructured tendon-derived scaffolds for enhanced bone regeneration by human adipose-derived stem cells

Eunkyung Ko, Kyle Alberti, Jong Seung Lee, Kisuk Yang, Yoonhee Jin, Jisoo Shin, Hee Seok Yang, Qiaobing Xu, Seung-Woo Cho

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Decellularized matrix-based scaffolds can induce enhanced tissue regeneration due to their biochemical, biophysical, and mechanical similarity to native tissues. In this study, we report a nanostructured decellularized tendon scaffold with aligned, nanofibrous structures to enhance osteogenic differentiation and in vivo bone formation of human adipose-derived stem cells (hADSCs). Using a bioskiving method, we prepared decellularized tendon scaffolds from tissue slices of bovine Achilles and neck tendons with or without fixation, and investigated the effects on physical and mechanical properties of decellularized tendon scaffolds, based on the types and concentrations of cross-linking agents. In general, we found that decellularized tendon scaffolds without fixative treatments were more effective in inducing osteogenic differentiation and mineralization of hADSCs in vitro. When non-cross-linked decellularized tendon scaffolds were applied together with hydroxyapatite for hADSC transplantation in critical-sized bone defects, they promoted bone-specific collagen deposition and mineralized bone formation 4 and 8 weeks after hADSC transplantation, compared to conventional collagen type I scaffolds. Interestingly, stacking of decellularized tendon scaffolds cultured with osteogenically committed hADSCs and those containing human cord blood-derived endothelial progenitor cells (hEPCs) induced vascularized bone regeneration in the defects 8 weeks after transplantation. Our study suggests that biomimetic nanostructured scaffolds made of decellularized tissue matrices can serve as functional tissue-engineering scaffolds for enhanced osteogenesis of stem cells.

Original languageEnglish
Pages (from-to)22819-22829
Number of pages11
JournalACS Applied Materials and Interfaces
Volume8
Issue number35
DOIs
Publication statusPublished - 2016 Sep 7

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Tendons
Stem cells
Scaffolds
Bone
Scaffolds (biology)
Tissue Scaffolds
Tissue
Collagen
Tissue regeneration
Fixatives
Defects
Endothelial cells
Biomimetics
Durapatite
Collagen Type I
Hydroxyapatite
Tissue engineering
Blood
Physical properties
Mechanical properties

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Ko, Eunkyung ; Alberti, Kyle ; Lee, Jong Seung ; Yang, Kisuk ; Jin, Yoonhee ; Shin, Jisoo ; Yang, Hee Seok ; Xu, Qiaobing ; Cho, Seung-Woo. / Nanostructured tendon-derived scaffolds for enhanced bone regeneration by human adipose-derived stem cells. In: ACS Applied Materials and Interfaces. 2016 ; Vol. 8, No. 35. pp. 22819-22829.
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Nanostructured tendon-derived scaffolds for enhanced bone regeneration by human adipose-derived stem cells. / Ko, Eunkyung; Alberti, Kyle; Lee, Jong Seung; Yang, Kisuk; Jin, Yoonhee; Shin, Jisoo; Yang, Hee Seok; Xu, Qiaobing; Cho, Seung-Woo.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 35, 07.09.2016, p. 22819-22829.

Research output: Contribution to journalArticle

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