Intracellular delivery of macromolecules is a significant technique in research and therapeutic applications. However, conventional vector-based and physical methods, which rely on exogenous material, high energy particle, or electrical field, can cause toxicity or off-target effects. To overcome this issue, we have targeted mechanical deformation of cellular membrane, which can lead to higher cellular uptake rate. Here, we demonstrated nano-villi platform that can mechanically stimulate cells and enhance intracellular delivery rate. By testing transfection efficiency with GFP expressing HeLa cells, it was found out that the transfection efficiency was significantly increased when nano-scale structures existed on the surface.
|Title of host publication||MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Publisher||Chemical and Biological Microsystems Society|
|Number of pages||3|
|Publication status||Published - 2015|
|Event||19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of|
Duration: 2015 Oct 25 → 2015 Oct 29
|Name||MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Other||19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015|
|Country/Territory||Korea, Republic of|
|Period||15/10/25 → 15/10/29|
Bibliographical noteFunding Information:
The authors acknowledge financial support from National Research Foundation (NRF-2014R1A1A1004985) and by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI14C00420200).
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering