Nanovilli based reverse uptake plarform for highly efficient intracellular delivery and transfection

Hyunwoo Joo, Jisoo Shin, Minjeong Jang, Junghwa Cha, Hoon Eui Jeong, Seung Woo Cho, Pilnam Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Intracellular delivery of macromolecules is a significant technique in research and therapeutic applications. However, conventional vector-based and physical methods, which rely on exogenous material, high energy particle, or electrical field, can cause toxicity or off-target effects. To overcome this issue, we have targeted mechanical deformation of cellular membrane, which can lead to higher cellular uptake rate. Here, we demonstrated nano-villi platform that can mechanically stimulate cells and enhance intracellular delivery rate. By testing transfection efficiency with GFP expressing HeLa cells, it was found out that the transfection efficiency was significantly increased when nano-scale structures existed on the surface.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1528-1530
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Joo, H., Shin, J., Jang, M., Cha, J., Jeong, H. E., Cho, S. W., & Kim, P. (2015). Nanovilli based reverse uptake plarform for highly efficient intracellular delivery and transfection. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1528-1530). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.